Synthesis of acetylene linked double-nucleobase nucleos(t)ide building blocks and polymerase construction of DNA containing cytosines in the major groove
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21425799
DOI
10.1021/jo200436j
Knihovny.cz E-resources
- MeSH
- Acetylene chemistry MeSH
- Adenine analogs & derivatives chemical synthesis chemistry MeSH
- Cytosine chemistry MeSH
- DNA-Directed DNA Polymerase chemistry metabolism MeSH
- DNA chemistry genetics metabolism MeSH
- Nucleic Acid Conformation * MeSH
- Models, Molecular MeSH
- Nucleosides chemical synthesis chemistry MeSH
- Nucleotides chemical synthesis chemistry MeSH
- Base Sequence MeSH
- Uracil chemical synthesis chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 7-deazaadenine MeSH Browser
- Acetylene MeSH
- Adenine MeSH
- Cytosine MeSH
- DNA-Directed DNA Polymerase MeSH
- DNA MeSH
- Nucleosides MeSH
- Nucleotides MeSH
- Uracil MeSH
(Cytosin-5-yl)ethynyl derivatives of pyrimidine and 7-deazaadenine 2-deoxyribonucleosides and nucleoside triphosphates (dNTPs) were prepared in one step by the aqueous Sonogashira coupling of unprotected halogenated nucleos(t)ides with 5-ethynylcytosine. The modified dNTPs were good substrates for DNA polymerases suitable for primer extension or PCR construction of DNA bearing acetylene-linked cytosine(s) in the major groove mimicking the flipped-out nucleotide.
References provided by Crossref.org
