Fermentation of mucin by bifidobacteria from rectal samples of humans and rectal and intestinal samples of animals
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- Bifidobacterium enzymologie genetika izolace a purifikace metabolismus MeSH
- dospělí MeSH
- fermentace MeSH
- glukosa metabolismus MeSH
- kojenec MeSH
- koncentrace vodíkových iontů MeSH
- kozy MeSH
- lidé MeSH
- muciny metabolismus MeSH
- polysacharid-lyasy chemie metabolismus MeSH
- skot MeSH
- stabilita enzymů MeSH
- teplota MeSH
- tlusté střevo mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- kojenec MeSH
- lidé MeSH
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- glukosa MeSH
- hyaluronate lyase MeSH Prohlížeč
- muciny MeSH
- polysacharid-lyasy MeSH
Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial strains of animal origin. The difference between human and animal isolates in ability to grow on mucin was significant at p < 0.001. Nine human strains with the best growth on mucin were identified as Bifidobacterium bifidum. These strains produced extracellular, membrane-bound, and intracellular mucinases with activities of 0.11, 0.53, and 0.09 μmol/min of reducing sugars per milligram of protein, respectively. Membrane-bound mucinases were active between pH 5 and 10. The optimum pH of extracellular mucinases was 6-7. Fermentation patterns in cultures grown on mucin and glucose differed. On mucin, the acetate-to-lactate ratio was higher than in cultures grown on glucose (p = 0.012). We showed that the bifidobacteria belong to the mucin-fermenting bacteria in humans, but their significance in mucin degradation in animals seems to be limited.
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Lett Appl Microbiol. 2002;35(2):126-30 PubMed
Antonie Van Leeuwenhoek. 1983 Sep;49(3):209-24 PubMed
Folia Microbiol (Praha). 2006;51(4):325-8 PubMed
Appl Environ Microbiol. 1984 Sep;48(3):626-32 PubMed
Gut. 1972 Aug;13(8):666-72 PubMed
J Microbiol Methods. 2005 Mar;60(3):365-73 PubMed
J Microbiol Methods. 2000 May;40(3):221-4 PubMed
J Bacteriol. 1968 Aug;96(2):472-8 PubMed
Appl Environ Microbiol. 2008 Mar;74(6):1936-40 PubMed
Folia Microbiol (Praha). 1993;38(5):383-6 PubMed
Appl Environ Microbiol. 1977 Nov;34(5):529-33 PubMed
Anaerobe. 2010 Apr;16(2):131-6 PubMed
J Biol Chem. 1984 Dec 10;259(23):14743-9 PubMed
Appl Environ Microbiol. 1999 Oct;65(10):4506-12 PubMed
J Exp Med. 1965 Feb 1;121:201-13 PubMed
J Biosci Bioeng. 2005 May;99(5):457-65 PubMed
Anal Biochem. 1977 Jul;81(1):21-7 PubMed
Infect Immun. 1992 Oct;60(10):3971-8 PubMed
Appl Environ Microbiol. 1982 Feb;43(2):325-30 PubMed
Gut. 1970 May;11(5):450-6 PubMed
Int J Food Microbiol. 1994 Dec;24(1-2):199-210 PubMed
Int J Syst Evol Microbiol. 2004 Sep;54(Pt 5):1469-1476 PubMed
