Fermentation of mucin by bifidobacteria from rectal samples of humans and rectal and intestinal samples of animals
Language English Country United States Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bifidobacterium enzymology genetics isolation & purification metabolism MeSH
- Adult MeSH
- Fermentation MeSH
- Glucose metabolism MeSH
- Infant MeSH
- Hydrogen-Ion Concentration MeSH
- Goats MeSH
- Humans MeSH
- Mucins metabolism MeSH
- Polysaccharide-Lyases chemistry metabolism MeSH
- Cattle MeSH
- Enzyme Stability MeSH
- Temperature MeSH
- Intestine, Large microbiology MeSH
- Animals MeSH
- Check Tag
- Adult MeSH
- Infant MeSH
- Humans MeSH
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Glucose MeSH
- hyaluronate lyase MeSH Browser
- Mucins MeSH
- Polysaccharide-Lyases MeSH
Bifidobacteria (246 strains in total) were isolated from rectal samples of infants and adult humans and animals, and from intestinal samples of calves. Twenty-five strains grew well on mucin: 20 from infants, two from adults, and three from goatlings. Poor or no growth on mucin was observed in 156 bifidobacterial strains of animal origin. The difference between human and animal isolates in ability to grow on mucin was significant at p < 0.001. Nine human strains with the best growth on mucin were identified as Bifidobacterium bifidum. These strains produced extracellular, membrane-bound, and intracellular mucinases with activities of 0.11, 0.53, and 0.09 μmol/min of reducing sugars per milligram of protein, respectively. Membrane-bound mucinases were active between pH 5 and 10. The optimum pH of extracellular mucinases was 6-7. Fermentation patterns in cultures grown on mucin and glucose differed. On mucin, the acetate-to-lactate ratio was higher than in cultures grown on glucose (p = 0.012). We showed that the bifidobacteria belong to the mucin-fermenting bacteria in humans, but their significance in mucin degradation in animals seems to be limited.
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