Activation of cumulus cell SMAD2/3 and epidermal growth factor receptor pathways are involved in porcine oocyte-cumulus cell expansion and steroidogenesis
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21520325
DOI
10.1002/mrd.21312
Knihovny.cz E-resources
- MeSH
- Benzamides pharmacology MeSH
- C-Reactive Protein metabolism MeSH
- Quinazolines pharmacology MeSH
- Dioxoles pharmacology MeSH
- Epidermal Growth Factor metabolism MeSH
- ErbB Receptors antagonists & inhibitors metabolism MeSH
- Follicle Stimulating Hormone metabolism MeSH
- Glucuronosyltransferase antagonists & inhibitors metabolism MeSH
- Isoquinolines pharmacology MeSH
- Cumulus Cells metabolism MeSH
- Hyaluronic Acid biosynthesis MeSH
- Meiosis drug effects MeSH
- Cell Adhesion Molecules antagonists & inhibitors metabolism MeSH
- Mice MeSH
- Oocytes enzymology physiology MeSH
- Swine MeSH
- Progesterone biosynthesis MeSH
- Smad2 Protein antagonists & inhibitors metabolism MeSH
- Smad3 Protein antagonists & inhibitors metabolism MeSH
- Pyridines pharmacology MeSH
- Pyrroles pharmacology MeSH
- Serum Amyloid P-Component metabolism MeSH
- Signal Transduction drug effects MeSH
- Tyrphostins pharmacology MeSH
- Gene Expression Regulation, Developmental drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide MeSH Browser
- 6,7-dimethyl-2-(2E)-3-(1-methyl-2-phenyl-1H-pyrrolo(2,3-b)pyridin-3-yl-prop-2-enoyl)-1,2,3,4-tetrahydroisoquinoline hydrochloride MeSH Browser
- Benzamides MeSH
- C-Reactive Protein MeSH
- Quinazolines MeSH
- Dioxoles MeSH
- Epidermal Growth Factor MeSH
- ErbB Receptors MeSH
- Follicle Stimulating Hormone MeSH
- Glucuronosyltransferase MeSH
- Isoquinolines MeSH
- Hyaluronic Acid MeSH
- Cell Adhesion Molecules MeSH
- Progesterone MeSH
- Smad2 Protein MeSH
- Smad3 Protein MeSH
- PTX3 protein MeSH Browser
- Pyridines MeSH
- Pyrroles MeSH
- RTKI cpd MeSH Browser
- Serum Amyloid P-Component MeSH
- Tyrphostins MeSH
Several lines of evidence suggest that in mice the activation of SMAD2/3 signaling by oocyte secreted factors, together with epidermal growth factor receptor (EGFR) activation, is essential to induce cumulus expansion. Here we show that inhibition of EGFR kinase in follicle stimulating hormone (FSH)-stimulated porcine oocyte-cumulus cell complex (OCCs) strongly decreases hyaluronan (HA) synthesis and its retention in the matrix, as well as progesterone synthesis. Although porcine cumulus cells undergo expansion independently of oocytes, we use biochemical and gene expression analyses to show that they do require activation of SMAD2/3 for optimal stimulation of HA synthesis and proteins involved in the organization of this polymer in the expanded matrix. Furthermore, FSH-induced progesterone synthesis by porcine cumulus cells was increased by blocking SMAD2/3 activation. In conclusion, these results support the hypothesis that an FSH-EGF autocrine loop is active in porcine OCCs, and provide the first evidence that the SMAD2/3 signaling pathway is induced by paracrine/autocrine factors in porcine cumulus cells and is involved in the control of both cumulus expansion and steroidogenesis.
References provided by Crossref.org
The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction
