Iron oxide nanoparticle coating of organic polymer-based monolithic columns for phosphopeptide enrichment
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
21560247
DOI
10.1002/jssc.201100256
Knihovny.cz E-zdroje
- Klíčová slova
- Enrichment, Iron oxide nanoparticles, Monolithic column, Phosphopeptides,
- MeSH
- chromatografie kapalinová přístrojové vybavení metody MeSH
- fosfopeptidy chemie izolace a purifikace MeSH
- kaseiny chemie MeSH
- nanočástice chemie MeSH
- polymery chemická syntéza chemie MeSH
- železité sloučeniny chemie MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ferric oxide MeSH Prohlížeč
- fosfopeptidy MeSH
- kaseiny MeSH
- polymery MeSH
- železité sloučeniny MeSH
A new monolithic capillary column with an iron oxide nanoparticle coating has been developed for selective and efficient enrichment of phosphopeptides. Iron oxide nanoparticles were prepared by a co-precipitation method and stabilized by citrate ions. A stable coating of nanoparticles was obtained via multivalent electrostatic interactions of citrate ions on the surface of iron oxide nanoparticles with a quaternary amine functionalized poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith. A high dynamic binding capacity of 86 μmol/mL column volume was measured with an adenosine-5'-triphosphate. Performance of the monolithic column was demonstrated with the efficient and selective enrichment of phosphopeptides from peptide mixtures of α-casein and β-casein digests and their MALDI/MS characterization in off-line mode.
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