In vitro effects of acetylcholinesterase inhibitors and reactivators on Complex I of electron transport chain
Jazyk angličtina Země Švédsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
21712782
PII: NEL320311A18
Knihovny.cz E-zdroje
- MeSH
- cholinesterasové inhibitory farmakologie MeSH
- elektronový transportní řetězec antagonisté a inhibitory účinky léků MeSH
- energetický metabolismus účinky léků MeSH
- mitochondrie účinky léků metabolismus MeSH
- mozek - chemie účinky léků MeSH
- oximy farmakologie MeSH
- prasata MeSH
- reaktivátory cholinesterasy farmakologie MeSH
- respirační komplex I metabolismus MeSH
- takrin analogy a deriváty farmakologie MeSH
- techniky in vitro MeSH
- transport elektronů účinky léků MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 7-methoxytacrine MeSH Prohlížeč
- cholinesterasové inhibitory MeSH
- elektronový transportní řetězec MeSH
- oximy MeSH
- reaktivátory cholinesterasy MeSH
- respirační komplex I MeSH
- takrin MeSH
OBJECTIVES: Inhibition of the enzyme acetylcholinesterase (AChE) is the main mechanism both of therapeutic action of drugs for the treatment of Alzheimer's disease and toxic action of organophosphorus compounds. Various types of oximes reactivate AChE and are commonly used as antidotes against organophosphates (pesticides, nerve agents). METHODS: Effects both of AChE inhibitors (tacrine, 7-methoxytacrine) and oximes (pralidoxime, trimedoxime, obidoxime, methoxime, HI-6) on Complex I of electron transport chain (ETC) were examined. The enzyme activity was measured spectrophotometrically in crude mitochondrial fraction isolated from pig brain. RESULTS: Our results showed statistically significant Complex I inhibition by tacrine, other drugs did not affect the enzyme activity significantly. CONCLUSIONS: These observations suggest the possibility of tacrine-induced side effects related to disturbance in ETC. On the contrary, it seems that oximes do not affect cellular energetic metabolism.
