Antioxidant activity of grape skin aqueous extracts from pressurized hot water extraction combined with electron paramagnetic resonance spectroscopy
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21872083
DOI
10.1016/j.talanta.2011.07.079
PII: S0039-9140(11)00672-2
Knihovny.cz E-resources
- MeSH
- Antioxidants chemistry MeSH
- Chemical Fractionation methods MeSH
- Electron Spin Resonance Spectroscopy methods MeSH
- Plant Epidermis chemistry MeSH
- Plant Extracts chemistry MeSH
- Spectrophotometry, Ultraviolet MeSH
- Pressure MeSH
- Vitis chemistry MeSH
- Water chemistry MeSH
- Hot Temperature * MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antioxidants MeSH
- Plant Extracts MeSH
- Water MeSH
Pressurized hot water extraction (PHWE) was employed to prepare extracts from dried grape skin of two wine grape varieties (St. Laurent and Alibernet) at various temperatures (from 40 up to 120°C) and amounts of sample (0.5, 1.0 and 1.5 g). To assess the antioxidant activity of the extracts, electron paramagnetic resonance (EPR) spectroscopy was applied involving DPPH and ABTS(+) assays. Other extract characteristics including HPLC profile of anthocyanins and total phenolic compound content were obtained as well. PHWE has also been compared with earlier results of extractions of the same grape skin samples with compressed methanol and compressed ethanol under the conditions of pressurized fluid extraction (PFE). From this comparison, PHWE emerges as the more benign and efficient extraction method to recover valuable phenolic antioxidants from grape skins for the prospective use in functional food supplements.
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