Microfluidic tool based on the antibody-modified paramagnetic particles for detection of 8-hydroxy-2'-deoxyguanosine in urine of prostate cancer patients
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- 8-Hydroxy-2'-Deoxyguanosine MeSH
- Alkaline Phosphatase MeSH
- Deoxyguanosine analogs & derivatives urine MeSH
- Electrochemical Techniques methods MeSH
- Enzyme-Linked Immunosorbent Assay methods MeSH
- Middle Aged MeSH
- Humans MeSH
- Magnets * MeSH
- Microfluidic Analytical Techniques instrumentation methods MeSH
- Microspheres MeSH
- Prostatic Neoplasms urine MeSH
- Naphthols MeSH
- Oxidative Stress MeSH
- Antibodies MeSH
- Flow Injection Analysis MeSH
- Robotics instrumentation methods MeSH
- Aged MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1-naphthol MeSH Browser
- 8-Hydroxy-2'-Deoxyguanosine MeSH
- Alkaline Phosphatase MeSH
- Deoxyguanosine MeSH
- Naphthols MeSH
- Antibodies MeSH
Guanosine derivatives are important for diagnosis of oxidative DNA damage including 8-hydroxy-2'-deoxyguanosine (8-OHdG) as one of the most abundant products of DNA oxidation. This compound is commonly determined in urine, which makes 8-OHdG a good non-invasive marker of oxidation stress. In this study, we optimized and tested the isolation of 8-OHdG from biological matrix by using paramagnetic particles with an antibody-modified surface. 8-OHdG was determined using 1-naphthol generated by alkaline phosphatase conjugated with the secondary antibody. 1-Naphthol was determined by stopped flow injection analysis (SFIA) with electrochemical detector using a glassy carbon working electrode and by stationary electrochemical detection using linear sweep voltammetry. A special modular electrochemical SFIA system which needs only 10 μL of sample including working buffer for one analysis was completely designed and successfully verified. The recoveries in different matrices and analyte concentration were estimated. Detection limit (3 S/N) was estimated as 5 pg/mL of 8-OHdG. This method promises to be very easily modified to microfluidic systems as "lab on valve". The optimized method had sufficient selectivity and thus could be used for determination of 8-OHDG in human urine and therefore for estimation of oxidative DNA damage as a result of oxidation stress in prostate cancer patients.
References provided by Crossref.org
Magnetic Nanoparticles: From Design and Synthesis to Real World Applications
ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation
