Engineered drought tolerance in tomato plants is reflected in chlorophyll fluorescence emission
Language English Country Ireland Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
22118618
DOI
10.1016/j.plantsci.2011.03.022
PII: S0168-9452(11)00099-9
Knihovny.cz E-resources
- MeSH
- Chlorophyll metabolism MeSH
- Dehydration genetics MeSH
- Adaptation, Physiological MeSH
- Stress, Physiological genetics MeSH
- Transcription, Genetic MeSH
- Genetic Variation MeSH
- Plants, Genetically Modified MeSH
- Plant Leaves growth & development MeSH
- Droughts * MeSH
- Plant Stomata physiology MeSH
- Gene Expression Regulation, Plant MeSH
- Genes, Plant MeSH
- Solanum lycopersicum genetics growth & development metabolism MeSH
- Gene Transfer Techniques MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Chlorophyll MeSH
Drought stress is one of the most important factors that limit crop productivity worldwide. In order to obtain tomato plants with enhanced drought tolerance, we inserted the transcription factor gene ATHB-7 into the tomato genome. This gene was demonstrated earlier to be up-regulated during drought stress in Arabidopsis thaliana thus acting as a negative regulator of growth. We compared the performance of wild type and transgenic tomato line DTL-20, carrying ATHB-7 gene, under well-irrigated and water limited conditions. We found that transgenic plants had reduced stomatal density and stomatal pore size and exhibited an enhanced resistance to soil water deficit. We used the transgenic plants to investigate the potential of chlorophyll fluorescence to report drought tolerance in a simulated high-throughput screening procedure. Wild type and transgenic tomato plants were exposed to drought stress lasting 18 days. The stress was then terminated by rehydration after which recovery was studied for another 2 days. Plant growth, leaf water potential, and chlorophyll fluorescence were measured during the entire experimental period. We found that water potential in wild type and drought tolerant transgenic plants diverged around day 11 of induced drought stress. The chlorophyll fluorescence parameters: the non-photochemical quenching, effective quantum efficiency of PSII, and the maximum quantum yield of PSII photochemistry yielded a good contrast between wild type and transgenic plants from day 7, day 12, and day 14 of induced stress, respectively. We propose that chlorophyll fluorescence emission reports well on the level of water stress and, thus, can be used to identify elevated drought tolerance in high-throughput screens for selection of resistant genotypes.
References provided by Crossref.org
Ectopic overexpression of the cell wall invertase gene CIN1 leads to dehydration avoidance in tomato