Oxidative damage to biological macromolecules in human bone marrow mesenchymal stromal cells labeled with various types of iron oxide nanoparticles
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22269213
DOI
10.1016/j.toxlet.2012.01.008
PII: S0378-4274(12)00024-0
Knihovny.cz E-resources
- MeSH
- Cell Death drug effects MeSH
- Child MeSH
- Middle Aged MeSH
- Humans MeSH
- Magnetic Resonance Imaging MeSH
- Magnetite Nanoparticles adverse effects MeSH
- Mesenchymal Stem Cells drug effects MeSH
- Oxidative Stress drug effects MeSH
- Lipid Peroxidation drug effects MeSH
- DNA Damage drug effects MeSH
- Proteins drug effects MeSH
- Cell Survival drug effects MeSH
- Ferric Compounds adverse effects MeSH
- Check Tag
- Child MeSH
- Middle Aged MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- ferric oxide MeSH Browser
- Magnetite Nanoparticles MeSH
- Proteins MeSH
- Ferric Compounds MeSH
The biological effects of several superparamagnetic iron oxide nanoparticles (SPIONs) varying in their surface coating were tested using human bone marrow mesenchymal stromal cells from two donors - hBMSCs-1 and hBMSCs-2. The measurements were performed at two intervals - after 72 h exposure to the nanoparticles and after an additional 72 h cell growth without nanoparticles. The dose of SPIONs used (15.4 μg Fe/ml) was selected as being sufficient for in vivo cell tracking using magnetic resonance imaging (MRI). Concerning cell viability and cell death, only the hBMSCs-2 seemed to be sensitive to the action of SPIONs. However, an increase of oxidative injury to lipids, proteins and DNA as a consequence of exposure to SPIONs was detected in cells from both donors. Particularly the levels of lipid peroxidation were high and increased further with time, regardless of the type of nanoparticle. Lowering intracellular label concentrations and authenticating oxidative stress levels using in vivo experiments are required to ensure the safety of SPIONs for biomedical applications.
References provided by Crossref.org
Biomaterials and Magnetic Stem Cell Delivery in the Treatment of Spinal Cord Injury
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Manganese-Zinc Ferrites: Safe and Efficient Nanolabels for Cell Imaging and Tracking In Vivo
Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
