Metabolic effects of n-3 PUFA as phospholipids are superior to triglycerides in mice fed a high-fat diet: possible role of endocannabinoids

. 2012 ; 7 (6) : e38834. [epub] 20120611

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid22701720

BACKGROUND: n-3 polyunsaturated fatty acids, namely docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), reduce the risk of cardiovascular disease and can ameliorate many of obesity-associated disorders. We hypothesised that the latter effect will be more pronounced when DHA/EPA is supplemented as phospholipids rather than as triglycerides. METHODOLOGY/PRINCIPAL FINDINGS: In a 'prevention study', C57BL/6J mice were fed for 9 weeks on either a corn oil-based high-fat obesogenic diet (cHF; lipids ∼35% wt/wt), or cHF-based diets in which corn oil was partially replaced by DHA/EPA, admixed either as phospholipids or triglycerides from marine fish. The reversal of obesity was studied in mice subjected to the preceding cHF-feeding for 4 months. DHA/EPA administered as phospholipids prevented glucose intolerance and tended to reduce obesity better than triglycerides. Lipemia and hepatosteatosis were suppressed more in response to dietary phospholipids, in correlation with better bioavailability of DHA and EPA, and a higher DHA accumulation in the liver, white adipose tissue (WAT), and muscle phospholipids. In dietary obese mice, both DHA/EPA concentrates prevented a further weight gain, reduced plasma lipid levels to a similar extent, and tended to improve glucose tolerance. Importantly, only the phospholipid form reduced plasma insulin and adipocyte hypertrophy, while being more effective in reducing hepatic steatosis and low-grade inflammation of WAT. These beneficial effects were correlated with changes of endocannabinoid metabolome in WAT, where phospholipids reduced 2-arachidonoylglycerol, and were more effective in increasing anti-inflammatory lipids such as N-docosahexaenoylethanolamine. CONCLUSIONS/SIGNIFICANCE: Compared with triglycerides, dietary DHA/EPA administered as phospholipids are superior in preserving a healthy metabolic profile under obesogenic conditions, possibly reflecting better bioavalability and improved modulation of the endocannabinoid system activity in WAT.

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Van Gaal LF, Mertens IL, De Block CE. Mechanisms linking obesity with cardiovascular disease. Nature. 2006;444:880. PubMed

Rustan AC, Christiansen EN, Drevon CA. Serum lipids, hepatic glycerolipid metabolism and peroxisomal fatty acid oxidation in rats fed omega-3 and omega-6 fatty acids. Biochem J 283 (Pt. 1992;2):339. PubMed PMC

Flachs P, Mohamed-Ali V, Horakova O, Rossmeisl M, Hosseinzadeh-Attar MJ. Polyunsaturated fatty acids of marine origin induce adiponectin in mice fed high-fat diet. Diabetologia. 2006;49:397. PubMed

Lankinen M, Schwab U, Erkkila A, Seppanen-Laakso T, Hannila ML. Fatty fish intake decreases lipids related to inflammation and insulin signaling–a lipidomics approach. PLoS ONE. 2009;4:e5258. PubMed PMC

Saravanan P, Davidson NC, Schmidt EB, Calder PC. Cardiovascular effects of marine omega-3 fatty acids. Lancet. 2010;376:550. PubMed

Todoric J, Loffler M, Huber J, Bilban M, Reimers M. Adipose tissue inflammation induced by high-fat diet in obese diabetic mice is prevented by n-3 polyunsaturated fatty acids. Diabetologia. 2006;49:2119. PubMed

Oh DY, Talukdar S, Bae EJ, Imamura T, Morinaga H. GPR120 Is an Omega-3 Fatty Acid Receptor Mediating Potent Anti-inflammatory and Insulin-Sensitizing Effects. Cell. 2010;142:698. PubMed PMC

Kuda O, Jelenik T, Jilkova Z, Flachs P, Rossmeisl M. n-3 Fatty acids and rosiglitazone improve insulin sensitivity through additive stimulatory effects on muscle glycogen synthesis in mice fed a high-fat diet. Diabetologia. 2009;52:951. PubMed

Flachs P, Ruhl R, Hensler M, Janovská P, Zouhar P. Synergistic induction of lipid catabolism and anti-inflammatory lipids in white fat of dietary obese mice in response to calorie restriction and n-3 fatty acids. Diabetologia. 2011;54:2638. PubMed

Jelenik T, Rossmeisl M, Kuda O, Jilkova ZM, Medrikova D. AMP-activated protein kinase {alpha}2 subunit is required for the preservation of hepatic insulin sensitivity by n-3 polyunsaturated fatty acids. Diabetes. 2010;59:2746. PubMed PMC

Tandy S, Chung RW, Wat E, Kamili A, Berge K. Dietary krill oil supplementation reduces hepatic steatosis, glycemia, and hypercholesterolemia in high-fat-fed mice. J Agric Food Chem. 2009;57:9345. PubMed

Rossmeisl M, Jelenik T, Jilkova Z, Slamova K, Kus V. Prevention and reversal of obesity and glucose intolerance in mice by DHA derivatives. Obesity. 2009;17:1031. PubMed

Nettleton JA, Katz R. n-3 long-chain polyunsaturated fatty acids in type 2 diabetes: a review. J Am Diet Assoc. 2005;105:440. PubMed

Kromhout D, Giltay EJ, Geleijnse JM Alpha Omega Trial Group. n-3 fatty acids and cardiovascular events after myocardial infarction. N Engl J Med 363: 2015–2026. 10.1056/NEJMoa1003603 [doi] 2010. PubMed

Kromhout D, Geleijnse JM, de Goede J, Oude Griep LM, Mulder BJ. n-3 Fatty Acids, Ventricular Arrhythmia-Related Events, and Fatal Myocardial Infarction in Postmyocardial Infarction Patients With Diabetes. Diabetes Care 34: 2515–2520. 34/12/2515 [pii];10.2337/dc11-0896 [doi] 2011. PubMed PMC

Mori TA, Bao DQ, Burke V, Puddey IB, Watts GF. Dietary fish as a major component of a weight-loss diet: effect on serum lipids, glucose, and insulin metabolism in overweight hypertensive subjects. Am J Clin Nutr. 1999;70:825. PubMed

Kunesova M, Braunerova R, Hlavaty P, Tvrzicka E, Stankova B. The influence of n-3 polyunsaturated fatty acids and very low calorie diet during a short-term weight reducing regimen on weight loss and serum fatty acid composition in severely obese women. Physiol Res. 2006;55:72. PubMed

Storlien LH, Kraegen EW, Chisholm DJ, Ford GL, Bruce DG. Fish oil prevents insulin resistance induced by high-fat feeding in rats. Science. 1987;237:888. PubMed

Friedberg CE, Janssen MJ, Heine RJ, Grobbee DE. Fish oil and glycemic control in diabetes. A meta-analysis. Diabetes Care. 1998;21:500. PubMed

Madsen L, Petersen RK, Kristiansen K. Regulation of adipocyte differentiation and function by polyunsaturated fatty acids. Biochim Biophys Acta. 2005;1740:286. PubMed

Neschen S, Morino K, Rossbacher JC, Pongratz RL, Cline GW. Fish Oil Regulates Adiponectin Secretion by a Peroxisome Proliferator-Activated Receptor-gamma-Dependent Mechanism in Mice. Diabetes. 2006;55:928. PubMed

Serhan CN. Novel omega – 3-derived local mediators in anti-inflammation and resolution. Pharmacol Ther. 2005;105:21. PubMed

Banni S, Di M V. Effect of dietary fat on endocannabinoids and related mediators: consequences on energy homeostasis, inflammation and mood. Mol Nutr Food Res. 2010;54:92. PubMed

Bluher M, Engeli S, Kloting N, Berndt J, Fasshauer M. Dysregulation of the peripheral and adipose tissue endocannabinoid system in human abdominal obesity. Diabetes 55: 3053–3060. 55/11/3053 [pii];10.2337/db06-0812 [doi] 2006. PubMed PMC

Batetta B, Griinari M, Carta G, Murru E, Ligresti A. Endocannabinoids may mediate the ability of (n-3) fatty acids to reduce ectopic fat and inflammatory mediators in obese Zucker rats. J Nutr. 2009;139:1501. PubMed

Piscitelli F, Carta G, Bisogno T, Murru E, Cordeddu L. Effect of dietary krill oil supplementation on the endocannabinoidome of metabolically relevant tissues from high fat-fed mice. Nutr Metab (Lond) 2011;8:51. PubMed PMC

Vaitheesvaran B, Yang L, Hartil K, Glaser S, Yazulla S. Peripheral Effects of FAAH Deficiency on Fuel and Energy Homeostasis: Role of Dysregulated Lysine Acetylation. PLoS ONE 7: e33717. 10.1371/journal.pone.0033717 [doi];PONE-D-11-20335 [pii] 2012. PubMed PMC

Chakravarthy MV, Lodhi IJ, Yin L, Malapaka RRV, Xu HE. Identification of a physiologically relevant endogenous ligand for PPARalfa in liver. Cell. 2009;138:488. PubMed PMC

Li Y, Choi M, Cavey G, Daugherty J, Suino K. Crystallographic identification and functional characterization of phospholipids as ligands for the orphan nuclear receptor steroidogenic factor-1. Molecular Cell. 2005;17:502. PubMed

Cansell M, Nacka F, Combe N. Marine lipid-based liposomes increase in vivo FA bioavailability. Lipids. 2003;38:559. PubMed

Cansell MS, Battin A, Degrace P, Gresti J, Clouet P. Early dissimilar fates of liver eicosapentaenoic Acid in rats fed liposomes or fish oil and gene expression related to lipid metabolism. Lipids. 2009;44:247. PubMed

Amate L, Gil A, Ramirez M. Feeding infant piglets formula with long-chain polyunsaturated fatty acids as triacylglycerols or phospholipids influences the distribution of these fatty acids in plasma lipoprotein fractions. J Nutr. 2001;131:1255. PubMed

Ulven SM, Kirkhus B, Lamglait A, Basu S, Elind E. Metabolic effects of krill oil are essentially similar to those of fish oil but at lower dose of EPA and DHA, in healthy volunteers. Lipids. 2011;46:46. PubMed PMC

Maki KC, Reeves MS, Farmer M, Griinari M, Berge K. Krill oil supplementation increases plasma concentrations of eicosapentaenoic and docosahexaenoic acids in overweight and obese men and women. Nutr Res. 2009;29:615. PubMed

Schuchardt JP, Schneider I, Meyer H, Neubronner J, von Schacky C. Incorporation of EPA and DHA into plasma phospholipids in response to different omega-3 fatty acid formulations–a comparative bioavailability study of fish oil vs. krill oil. Lipids Health Dis 10: 145. 1476-511X-10-145 [pii];10.1186/1476-511X-10-145 [doi] 2011. PubMed PMC

Tvrzicka E, Vecka M, Stankova B, Zak A. Analysis of fatty acids in plasma lipoproteins by gas chromatography-flame ionization detection. Quantitative aspects. Analytical Chimica Acta. 2002;465:350.

Sjovall P, Johansson B, Belazi D, Stenvinkel P, Lindholm B. TOF-SIMS analysis of adipose tissue from patients with chronic kidney disease. Applied Surface Science. 2008;255:1180.

Magnusson YK, Friberg P, Sjovall P, Malm J, Chen Y. TOF-SIMS Analysis of Lipid Accumulation in the Skeletal Muscle of ob/ob Mice. Obesity (Silver Spring) 2008;16:2753. PubMed

Zierenberg O, Grundy SM. Intestinal absorption of polyenephosphatidylcholine in man. J Lipid Res. 1982;23:1142. PubMed

Rampone AJ, Long LW. The effect of phosphatidylcholine and lysophosphatidylcholine on the absorption and mucosal metabolism of oleic acid and cholesterol in vitro. Biochim Biophys Acta. 1977;486:510. PubMed

Arterburn LM, Hall EB, Oken H. Distribution, interconversion, and dose response of n-3 fatty acids in humans. Am J Clin Nutr. 2006;83:1476S. PubMed

Kopecky J, Rossmeisl M, Flachs P, Kuda O, Brauner P. n-3 PUFA: bioavailability and modulation of adipose tissue function. Proc Nutr Soc. 2009;68:369. PubMed

Pacetti D, Boselli E, Hulan HW, Frega NG. High performance liquid chromatography-tandem mass spectrometry of phospholipid molecular species in eggs from hens fed diets enriched in seal blubber oil. J Chromatogr A 1097: 66–73. S0021-9673(05)01639-0 [pii];10.1016/j.chroma.2005.08.016 [doi] 2005. PubMed

Pacetti D, Hulan HW, Schreiner M, Boselli E, Frega NG. Positional analysis of egg triccylglycerols and phospolipids from hens fed diets enriched with rafined seal blubber oil. J Sci Food Agric. 2005;85:1714.

Flachs P, Rossmeisl M, Bryhn M, Kopecky J. Cellular and molecular effects of n-3 polyunsaturated fatty acids on adipose tissue biology and metabolism. Clinical Sciences. 2009;116:16. PubMed

Calder PC. n-3 polyunsaturated fatty acids, inflammation, and inflammatory diseases. Am J Clin Nutr. 2006;83:1519S. PubMed

Miranville A, Herling AW, Biemer-Daub G, Voss MD. Reversal of inflammation-induced impairment of glucose uptake in adipocytes by direct effect of CB1 antagonism on adipose tissue macrophages. Obesity (Silver Spring) 2010;18:2254. PubMed

Osei-Hyiaman D, Liu J, Zhou L, Godlewski G, Harvey-White J. Hepatic CB1 receptor is required for development of diet-induced steatosis, dyslipidemia, and insulin and leptin resistance in mice. J Clin Invest. 2008;118:3169. PubMed PMC

Jourdan T, Djaouti L, Demizieux L, Gresti J, Verges B. CB1 Antagonism Exerts Specific Molecular Effects on Visceral and Subcutaneous Fat and Reverses Liver Steatosis in Diet-Induced Obese Mice. Diabetes. 2010;59:934. PubMed PMC

Balvers MGJ, Verhoeckx KCM, Plastina P, Wortelboer HM, Meijerink J. Docosahexaenoic acid and eicosapentaenoic acid are converted by 3T3-L1 adipocytes to N-acyl ethanolamines with anti-inflammatory properties. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 2010;1801:1114. PubMed

Meijerink J, Plastina P, Vincken JP, Poland M, Attya M. The ethanolamide metabolite of DHA, docosahexaenoylethanolamine, shows immunomodulating effects in mouse peritoneal and RAW264.7 macrophages: evidence for a new link between fish oil and inflammation. Br J Nutr 1–10. 2011. PubMed

Lambert DM, Muccioli GG. Endocannabinoids and related N-acylethanolamines in the control of appetite and energy metabolism: emergence of new molecular players. Curr Opin Clin Nutr Metab Care 10: 735–744. 10.1097/MCO.0b013e3282f00061 [doi];00075197-200711000-00014 [pii] 2007. PubMed

Blasbalg TL, Hibbeln JR, Ramsden CE, Majchrzak SF, Rawlings RR. Changes in consumption of omega-3 and omega-6 fatty acids in the United States during the 20th century. Am J Clin Nutr 93: 950–962. ajcn.110.006643 [pii];10.3945/ajcn.110.006643 [doi] 2011. PubMed PMC

Alvheim AR, Malde MK, Osei-Hyiaman D, Hong LY, Pawlosky RJ. Dietary Linoleic Acid Elevates Endogenous 2-AG and Anandamide and Induces Obesity. Obesity (Silver Spring). oby201238 [pii];10.1038/oby.2012.38 [doi] 2012. PubMed PMC

Ruzickova J, Rossmeisl M, Prazak T, Flachs P, Sponarova J. Omega-3 PUFA of marine origin limit diet-induced obesity in mice by reducing cellularity of adipose tissue. Lipids. 2004;39:1185. PubMed

Sato A, Kawano H, Notsu T, Ohta M, Nakakuki M. Antiobesity effect of eicosapentaenoic acid in high-fat/high-sucrose diet-induced obesity: importance of hepatic lipogenesis. Diabetes. 2010;59:2504. PubMed PMC

Hao Q, Lillefosse HH, Fjaere E, Myrmel LS, Midtbo LK. High glycemic index carbohydrates abrogate the anti-obesity effect of fish oil in mice. Am J Physiol Endocrinol Metab. ajpendo.00524.2011 [pii];10.1152/ajpendo.00524.2011 [doi] 2012. PubMed

Kus V, Flachs P, Kuda O, Bardova K, Janovska P. Unmasking Differential Effects of Rosiglitazone and Pioglitazone in the Combination Treatment with n-3 Fatty Acids in Mice Fed a High-Fat Diet. Plos One. 2011;6:e27126. PubMed PMC

Butler H, Korbonits M. Cannabinoids for clinicians: the rise and fall of the cannabinoid antagonists. Eur J Endocrinol. 2009;161:662. PubMed PMC

Schuchardt JP, Neubronner J, Kressel G, Merkel M, von Schacky C. Moderate doses of EPA and DHA from re-esterified triacylglycerols but not from ethyl-esters lower fasting serum triacylglycerols in statin-treated dyslipidemic subjects: Results from a six month randomized controlled trial. Prostaglandins Leukot Essent Fatty Acids 85: 381–386. S0952-3278(11)00101-3 [pii];10.1016/j.plefa.2011.07.006 [doi] 2011. PubMed

Medrikova D, Macek JZ, Bardova K, Janovská P, Rossmeisl M. Sex differences during the course of diet-induced obesity in mice: adipose tissue expandability and glycemic control. Int J Obes. 2011;36:272. PubMed

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