Challenges in the development of bioanalytical liquid chromatography-mass spectrometry method with emphasis on fast analysis
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
22999195
DOI
10.1016/j.chroma.2012.08.087
PII: S0021-9673(12)01338-6
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Chromatography, Liquid economics instrumentation methods MeSH
- Mass Spectrometry economics instrumentation methods MeSH
- Humans MeSH
- Validation Studies as Topic MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
The development of bioanalytical methods has become more and more challenging over the past years due to very demanding requirements in terms of method reliability, sensitivity, speed of analysis and sample throughput. LC-MS/MS has established itself as a method of choice for routine analysis of biological materials. A development of such method consists of several steps including sample preparation and clean-up step, efficient chromatographic separation, sensitive and selective detection of analytes in complex matrices, a choice of convenient data processing and calibration approach and finally method validation. Each of these steps has its own constraints and challenges, which are discussed in detail in this review. Novel and modern approaches in sample preparation, chromatography and detection are especially emphasized. Attention is paid to proper calibration approach and matrix effects that can seriously affect method accuracy and precision.
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