Pseudoscardovia suis gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of wild pigs (Sus scrofa)
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23122702
DOI
10.1016/j.syapm.2012.09.001
PII: S0723-2020(12)00124-5
Knihovny.cz E-zdroje
- MeSH
- Actinobacteria chemie klasifikace genetika izolace a purifikace MeSH
- bakteriální proteiny genetika MeSH
- DNA bakterií chemie genetika MeSH
- fosfolipidy analýza MeSH
- fylogeneze MeSH
- gastrointestinální trakt mikrobiologie MeSH
- mastné kyseliny analýza MeSH
- molekulární sekvence - údaje MeSH
- peptidoglykan chemie MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza DNA MeSH
- shluková analýza MeSH
- Sus scrofa MeSH
- techniky typizace bakterií MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- DNA bakterií MeSH
- fosfolipidy MeSH
- mastné kyseliny MeSH
- peptidoglykan MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
Seventeen fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from the digestive tract of wild pigs (Sus scrofa). Most of them were identified as Bifidobacterium boum according to sequences of 16S rRNA gene. Two strains isolated from the small intestine content had unusual morphology of cells in comparison with bifidobacteria. Cells growing in liquid anaerobic media were regular shaped rods arranged mostly in pairs. These isolates showed relatively low 16S rRNA gene sequence similarities (maximum identity of 94%) to members of the family Bifidobacteriaceae. Nevertheless, phylogenetic analyses of 16S rRNA, hsp60 and xfp gene sequences revealed that these strains are more related to recently described Neoscardovia, Aeriscardovia and other scardovial genera, than to Bifidobacterium species. Partial gene sequences of other phylogenetic markers showed low (65.8-89.5%) similarities to genome sequences of bifidobacteria and Gardnerella vaginalis. The major fatty acids detected in cells of the representative strain DPTE4(T) were C(16:0), C(18:1), C(14:0). The peptidoglycan type of the DPTE4(T) strain was A3βl-Orn(l-Lys)-l-Ser(l-Ala)-l-Ala(2). Polar lipid analysis revealed two phosphoglycolipids and phospholipids, a glycolipid and diphosphatidylglycerol. The results of phylogenetic, genotypic and phenotypic analyses support the proposal of a novel taxa, Pseudoscardovia suis gen. nov., sp. nov. (type strain=DPTE4(T)=DSM 24744(T)=CCM 7942(T)).
Citace poskytuje Crossref.org
GENBANK
HQ842701, HQ842703, HQ851018, HQ851019, JQ363630, JQ363644, JQ363648, JQ363649, JQ363651, JQ363652, JQ363657, JQ363661, JQ363663, JQ363664, JQ363668, JQ363669