Antibacterial activity and cell viability of hyaluronan fiber with silver nanoparticles
Language English Country Great Britain, England Media print-electronic
Document type Journal Article
PubMed
23399144
DOI
10.1016/j.carbpol.2012.08.098
PII: S0144-8617(12)00889-2
Knihovny.cz E-resources
- MeSH
- Anti-Bacterial Agents chemistry pharmacology toxicity MeSH
- 3T3 Cells MeSH
- Escherichia coli drug effects MeSH
- Metal Nanoparticles chemistry MeSH
- Hyaluronic Acid chemistry pharmacology toxicity MeSH
- Mice MeSH
- Solvents chemistry MeSH
- Staphylococcus aureus drug effects MeSH
- Silver chemistry MeSH
- Particle Size MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Hyaluronic Acid MeSH
- Solvents MeSH
- Silver MeSH
Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.
References provided by Crossref.org
Self-Assembled Hydrogel Membranes with Structurally Tunable Mechanical and Biological Properties
Marine Biomaterials: Hyaluronan
