Changes of DNA methylation and hydroxymethylation in plant protoplast cultures
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23505619
PII: 2012_312
Knihovny.cz E-resources
- MeSH
- Cells, Cultured MeSH
- DNA Methylation * MeSH
- Protoplasts chemistry MeSH
- Reactive Oxygen Species MeSH
- Plants chemistry MeSH
- Cell Survival MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Reactive Oxygen Species MeSH
Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found that 5-hmC did not primarily arise as a product of oxidative DNA damage following protoplast culture.
Exploring the crop epigenome: a comparison of DNA methylation profiling techniques
