The dynamics of supernumerary tooth development are differentially regulated by Sprouty genes
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23606267
DOI
10.1002/jez.b.22502
Knihovny.cz E-resources
- MeSH
- Apoptosis genetics MeSH
- Biological Evolution * MeSH
- Epithelium growth & development MeSH
- Intracellular Signaling Peptides and Proteins genetics metabolism MeSH
- Membrane Proteins genetics metabolism MeSH
- Molar growth & development metabolism MeSH
- Mutation MeSH
- Mice MeSH
- Odontogenesis MeSH
- Protein Serine-Threonine Kinases MeSH
- Nerve Tissue Proteins genetics metabolism MeSH
- Incisor growth & development metabolism MeSH
- Signal Transduction MeSH
- Tooth, Supernumerary pathology MeSH
- Tooth growth & development MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Intracellular Signaling Peptides and Proteins MeSH
- Membrane Proteins MeSH
- Protein Serine-Threonine Kinases MeSH
- Nerve Tissue Proteins MeSH
- Spry2 protein, mouse MeSH Browser
- Spry4 protein, mouse MeSH Browser
In mice, a toothless diastema separates the single incisor from the three molars in each dental quadrant. In the prospective diastema of the embryo, small rudimentary buds are found that are presumed to be rudiments of suppressed teeth. A supernumerary tooth occurs in the diastema of adult mice carrying mutations in either Spry2 or Spry4. In the case of Spry2 mutants, the origin of the supernumerary tooth involves the revitalization of a rudimentary tooth bud (called R2), whereas its origin in the Spry4 mutants is not known. In addition to R2, another rudimentary primordium (called MS) arises more anteriorly in the prospective diastema. We investigated the participation of both rudiments (MS and R2) in supernumerary tooth development in Spry2 and Spry4 mutants by comparing morphogenesis, proliferation, apoptosis, size and Shh expression in the dental epithelium of MS and R2 rudiments. Increased proliferation and decreased apoptosis were found in MS and R2 at embryonic day (ED) 12.5 and 13.5 in Spry2(-/-) embryos. Apoptosis was also decreased in both rudiments in Spry4(-/-) embryos, but the proliferation was lower (similar to WT mice), and supernumerary tooth development was accelerated, exhibiting a cap stage by ED13.5. Compared to Spry2(-/-) mice, a high number of Spry4(-/-) supernumerary tooth primordia degenerated after ED13.5, resulting in a low percentage of supernumerary teeth in adults. We propose that Sprouty genes were implicated during evolution in reduction of the cheek teeth in Muridae, and their deletion can reveal ancestral stages of murine dental evolution.
References provided by Crossref.org
Role of Cell Death in Cellular Processes During Odontogenesis
Developmental variability channels mouse molar evolution
Modeling Edar expression reveals the hidden dynamics of tooth signaling center patterning
Sprouty gene dosage influences temporal-spatial dynamics of primary enamel knot formation
Three-dimensional analysis of the early development of the dentition
