The dynamics of supernumerary tooth development are differentially regulated by Sprouty genes
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23606267
DOI
10.1002/jez.b.22502
Knihovny.cz E-zdroje
- MeSH
- apoptóza genetika MeSH
- biologická evoluce * MeSH
- epitel růst a vývoj MeSH
- intracelulární signální peptidy a proteiny genetika metabolismus MeSH
- membránové proteiny genetika metabolismus MeSH
- moláry růst a vývoj metabolismus MeSH
- mutace MeSH
- myši MeSH
- odontogeneze MeSH
- protein-serin-threoninkinasy MeSH
- proteiny nervové tkáně genetika metabolismus MeSH
- řezáky růst a vývoj metabolismus MeSH
- signální transdukce MeSH
- zuby přespočetné patologie MeSH
- zuby růst a vývoj MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- intracelulární signální peptidy a proteiny MeSH
- membránové proteiny MeSH
- protein-serin-threoninkinasy MeSH
- proteiny nervové tkáně MeSH
- Spry2 protein, mouse MeSH Prohlížeč
- Spry4 protein, mouse MeSH Prohlížeč
In mice, a toothless diastema separates the single incisor from the three molars in each dental quadrant. In the prospective diastema of the embryo, small rudimentary buds are found that are presumed to be rudiments of suppressed teeth. A supernumerary tooth occurs in the diastema of adult mice carrying mutations in either Spry2 or Spry4. In the case of Spry2 mutants, the origin of the supernumerary tooth involves the revitalization of a rudimentary tooth bud (called R2), whereas its origin in the Spry4 mutants is not known. In addition to R2, another rudimentary primordium (called MS) arises more anteriorly in the prospective diastema. We investigated the participation of both rudiments (MS and R2) in supernumerary tooth development in Spry2 and Spry4 mutants by comparing morphogenesis, proliferation, apoptosis, size and Shh expression in the dental epithelium of MS and R2 rudiments. Increased proliferation and decreased apoptosis were found in MS and R2 at embryonic day (ED) 12.5 and 13.5 in Spry2(-/-) embryos. Apoptosis was also decreased in both rudiments in Spry4(-/-) embryos, but the proliferation was lower (similar to WT mice), and supernumerary tooth development was accelerated, exhibiting a cap stage by ED13.5. Compared to Spry2(-/-) mice, a high number of Spry4(-/-) supernumerary tooth primordia degenerated after ED13.5, resulting in a low percentage of supernumerary teeth in adults. We propose that Sprouty genes were implicated during evolution in reduction of the cheek teeth in Muridae, and their deletion can reveal ancestral stages of murine dental evolution.
Citace poskytuje Crossref.org
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