Proteomic techniques for characterisation of mesenchymal stem cell secretome
Language English Country France Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
23880644
DOI
10.1016/j.biochi.2013.07.015
PII: S0300-9084(13)00237-X
Knihovny.cz E-resources
- Keywords
- 2-DE, 2-dimensional gel electrophoresis, ASCs, CM, CSC, Conditioned medium, ER, Exosome, MS, MSCs, Mesenchymal stem cells, Proteomics, SRM, Secretome, TEM, TNF, adipose stem/stromal cells, cell surface capturing, conditioned medium, endoplasmic reticulum, mass spectrometry, mesenchymal stem cells, selected reaction monitoring, transmission electron microscopy, tumour necrosis factor,
- MeSH
- Electrophoresis, Gel, Two-Dimensional MeSH
- Cell Differentiation MeSH
- Cell Culture Techniques MeSH
- Bone Marrow Cells cytology MeSH
- Exosomes chemistry MeSH
- Mass Spectrometry MeSH
- Culture Media, Conditioned MeSH
- Humans MeSH
- Mesenchymal Stem Cells metabolism MeSH
- Proteomics methods MeSH
- Adipose Tissue cytology MeSH
- Computational Biology methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Culture Media, Conditioned MeSH
Mesenchymal stem cells (MSCs) are multipotent cells with a substantial potential in human regenerative medicine due to their ability to migrate to sites of injury, capability to suppress immune response and accessibility in large amount from patient's own bone marrow or fat tissue. It has been increasingly observed that the transplanted MSCs did not necessarily engraft and differentiate at the site of injury but might exert their therapeutic effects through secreted trophic signals. The MSCs secrete a variety of autocrine/paracrine factors, called secretome, that support regenerative processes in the damaged tissue, induce angiogenesis, protect cells from apoptotic cell death and modulate immune system. The cell culture medium conditioned by MSCs or osteogenic, chondrogenic as well as adipogenic precursors derived from MSCs has become a subject of intensive proteomic profiling in the search for and identification of released factors and microvesicles that might be applicable in regenerative medicine. Jointly with the methods for MSC isolation, expansion and differentiation, proteomic analysis of MSC secretome was enabled recently mainly due to the extensive development in protein separation techniques, mass spectrometry, immunological methods and bioinformatics. This review describes proteomic techniques currently applied or prospectively applicable in MSC secretomics, with a particular focus on preparation of the secretome sample, protein/peptide separation, mass spectrometry and protein quantification techniques, analysis of posttranslational modifications, immunological techniques, isolation and characterisation of secreted vesicles and exosomes, analysis of cytokine-encoding mRNAs and bioinformatics.
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