Fungal microsomes in a biotransformation perspective: protein nature of membrane-associated reactions
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
- MeSH
- Biotransformation MeSH
- Fungi enzymology metabolism MeSH
- Intracellular Membranes enzymology metabolism MeSH
- Membrane Proteins metabolism MeSH
- Microsomes enzymology metabolism MeSH
- Proteomics methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Membrane Proteins MeSH
Microsomal fraction of fungal cells grabs the attention of many researchers for it contains enzymes that play a role in biotechnologically relevant processes. Microsomal enzymes, namely, CYP450s, were shown to metabolize a wide range of xenobiotic compounds, including PAHs, PCBs, dioxins, and endocrine disruptors, and take part in other fungal biotransformation reactions. However, little is known about the nature and regulation of these membrane-associated reactions. Advanced proteomic and post-genomic techniques make it possible to identify larger numbers of microsomal proteins and thus add to a deeper study of fungal intracellular processes. In this work, proteins that were identified through a shotgun proteomic approach in fungal microsomes under various culture conditions are reviewed. However, further research is still needed to fully understand the role of microsomes in fungal biodegradation and biotransformation reactions.
References provided by Crossref.org
A two-dimensional protein map of Pleurotus ostreatus microsomes-proteome dynamics
