HRS60.1, a monomer unit (184 bp) of a highly repeated nuclear DNA sequence of Nicotiana tabacum, has been cloned and sequenced. Following BamHI digestion of tobacco DNA, Southern hybridization with HRS60.1 revealed a ladder of hybridization bands corresponding to multiples of the basic monomer unit. If the tobacco DNA was digested with restriction endonucleases which have no target site in HRS60.1, the larger part of DNA homologous to HRS60.1 remained as uncleaved "relic" DNA. These results suggest a tandem arrangement of this DNA repeat unit. Four other clones of tobacco nuclear DNA cross-hybridized with HRS60.1, thus forming a "HRS60-family". Sequencing their inserts has shown their strong mutual homology. HRS60-family comprised about 2% of the nuclear genome of N. tabacum. Computer comparisons with other tandem plant-repeated DNA sequences could not detect any other homologous sequence.

Institute of Biophysics Czechoslovak Academy of Sciences CS 612 65 Brno Czechoslovakia

Zobrazit více v PubMed

Nucleic Acids Res. 1986 Oct 24;14(20):8111-9 PubMed

Nucleic Acids Res. 1984 Aug 24;12(16):6415-26 PubMed

Proc Natl Acad Sci U S A. 1981 Jul;78(7):4490-4 PubMed

Folia Biol (Praha). 1987;33(1):40-9 PubMed

J Mol Biol. 1983 Jun 5;166(4):557-80 PubMed

Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PubMed

Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PubMed

Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 PubMed

J Mol Biol. 1986 Feb 20;187(4):495-507 PubMed

Gene. 1985;33(1):103-19 PubMed

Cell. 1980 Feb;19(2):545-60 PubMed

Proc Natl Acad Sci U S A. 1981 Jan;78(1):338-42 PubMed

Plant Mol Biol. 1987 Nov;8(6):497-507 PubMed

Nucleic Acids Res. 1987 Aug 11;15(15):5913-23 PubMed

J Mol Biol. 1975 Nov 5;98(3):503-17 PubMed

Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PubMed

Telomeres and Their Neighbors

Three TERT genes in Nicotiana tabacum

Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells

Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco

Faithful inheritance of cytosine methylation patterns in repeated sequences of the allotetraploid tobacco correlates with the expression of DNA methyltransferase gene families from both parental genomes

The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco

Comparative analysis of DNA methylation in tobacco heterochromatic sequences

Evidence for a sequence-directed conformation periodicity in the genomic highly repetitive DNA detectable with single-strand-specific chemical probe potassium permanganate

Meiotic transmission of a hypomethylated repetitive DNA family in tobacco

Characterization of a new family of tobacco highly repetitive DNA, GRS, specific for the Nicotiana tomentosiformis genomic component

5-Azacytidine-induced hypomethylation of tobacco HRS60 tandem DNA repeats in tissue culture

Isolation and characterization of two middle repetitive DNA sequences of nuclear tobacco genome