Morphological analysis of embryonic cerebellar grafts in SCA2 mice
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24269873
DOI
10.1016/j.neulet.2013.11.020
PII: S0304-3940(13)01020-3
Knihovny.cz E-resources
- Keywords
- ATXN2(Q127), Cerebellar degeneration, DNA, Deoxyribonucleic acid, EGFP, GAFP, Neurotransplantation, PC, PCR, Purkinje cell, Purkinje cell degeneration, Purkinje cells, SCA, ataxin 2 with 127 glutamine repeats, enhanced green fluorescent protein, glial fibrillary acidic protein, pcd, polymerase chain reaction, spinocerebellar ataxia,
- MeSH
- Cerebellum pathology transplantation MeSH
- Mice, Transgenic MeSH
- Graft Survival MeSH
- Sex Factors MeSH
- Spinocerebellar Ataxias therapy MeSH
- Fetal Tissue Transplantation * MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
SCA2 transgenic mice are thought to be a useful model of human spinocerebellar ataxia type 2. There is no effective therapy for cerebellar degenerative disorders, therefore neurotransplantation could offer hope. The aim of this work was to assess the survival and morphology of embryonic cerebellar grafts transplanted into the cerebellum of adult SCA2 mice. Four month-old homozygous SCA2 and negative control mice were treated with bilateral intracerebellar injections of an enhanced green fluorescent protein-positive embryonic cerebellar cell suspension. Graft survival and morphology were examined three months later. Graft-derived Purkinje cells and the presence of astrocytes in the graft were detected immunohistochemically. Nissl and hematoxylin-eosin techniques were used to visualize the histological structure of the graft and surrounding host tissue. Grafts survived in all experimental mice; no differences in graft structure, between SCA2 homozygous and negative mice, were found. The grafts contained numerous Purkinje cells but long distance graft-to-host axonal connections to the deep cerebellar nuclei were rarely seen. Relatively few astrocytes were found in the center of the graft. No signs of inflammation or tissue destruction were seen in the area around the grafts. Despite good graft survival and the presence of graft-derived Purkinje cells, the structure of the graft did not seem to promise any significant specific functional effects. We have shown that the graft is available for long-term experiments. Nevertheless, it would be beneficial to search for ways of enhancement of connections between the graft and host.
References provided by Crossref.org
Quantification of Solid Embryonic Cerebellar Graft Volume in a Degenerative Ataxia Model
Long-Term Development of Embryonic Cerebellar Grafts in Two Strains of Lurcher Mice
Experimental neurotransplantation treatment for hereditary cerebellar ataxias
Transplantation of Embryonic Cerebellar Grafts Improves Gait Parameters in Ataxic Lurcher Mice
