Rapid matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging with scanning desorption laser beam
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24364764
DOI
10.1021/ac402823n
Knihovny.cz E-zdroje
- MeSH
- adrenokortikotropní hormon analýza MeSH
- design vybavení MeSH
- gentisáty chemie MeSH
- lasery MeSH
- lidé MeSH
- peptidy analýza chemická syntéza MeSH
- počítačové zpracování obrazu * MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice přístrojové vybavení metody MeSH
- světlo MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 2,5-dihydroxybenzoic acid MeSH Prohlížeč
- adrenokortikotropní hormon MeSH
- gentisáty MeSH
- peptidy MeSH
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 × 100 pixel MS image in ~11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
Citace poskytuje Crossref.org
MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
