Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes
Jazyk angličtina Země Rakousko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Arabidopsis genetika MeSH
- duplicitní geny * MeSH
- ekotyp * MeSH
- fyziologická adaptace účinky léků genetika MeSH
- kořeny rostlin anatomie a histologie účinky léků genetika růst a vývoj MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- měď metabolismus toxicita MeSH
- metalothionein 3 MeSH
- molekulární sekvence - údaje MeSH
- peroxid vodíku metabolismus MeSH
- proteiny nervové tkáně genetika MeSH
- regulace genové exprese u rostlin účinky léků MeSH
- rostlinné geny * MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- Silene genetika fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- měď MeSH
- metalothionein 3 MeSH
- peroxid vodíku MeSH
- proteiny nervové tkáně MeSH
Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress. We demonstrated that higher copper concentrations specifically affected MT3 expression among ecotypes. Our analysis shows that MT3a has similar expression pattern in cupricolous ecotypes while MT3b has common expression features shared by all metallophyte S. vulgaris ecotypes. Our data indicate that down-regulation of MT3b root expression in higher copper concentrations is associated with copper stress. We propose that there might be a specific regulation of SvMT3s transcription depending on the type of heavy metal tolerance.
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GENBANK
JX648329, JX648330, JX648331, JX648332, JX648333
