Expression response of duplicated metallothionein 3 gene to copper stress in Silene vulgaris ecotypes
Language English Country Austria Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Arabidopsis genetics MeSH
- Genes, Duplicate * MeSH
- Ecotype * MeSH
- Adaptation, Physiological drug effects genetics MeSH
- Plant Roots anatomy & histology drug effects genetics growth & development MeSH
- Real-Time Polymerase Chain Reaction MeSH
- Copper metabolism toxicity MeSH
- Metallothionein 3 MeSH
- Molecular Sequence Data MeSH
- Hydrogen Peroxide metabolism MeSH
- Nerve Tissue Proteins genetics MeSH
- Gene Expression Regulation, Plant drug effects MeSH
- Genes, Plant * MeSH
- Saccharomyces cerevisiae metabolism MeSH
- Silene genetics physiology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Copper MeSH
- Metallothionein 3 MeSH
- Hydrogen Peroxide MeSH
- Nerve Tissue Proteins MeSH
Metallothioneins (MTs) were identified as important players in metal metabolism. MT3 gene presents a key metallothionein controlling copper homeostasis in plants. We have selected one cupricolous and one non-cupricolous ecotype to isolate and analyse the MT3 gene in Silene vulgaris. For expression data comparison, we have also included other metal-tolerant ecotypes. Based on a S. vulgaris BAC library screening, we have identified and sequenced a genomic clone containing MT3 gene (SvMT3). We found that SvMT3 gene has been locally duplicated in a tandem arrangement. Expression analysis and complementation studies using yeast mutants showed that both copies of the SvMT3 gene were functional. Moreover, we examined the expression of MT3 gene(s) in selected ecotypes under different copper treatments to show the tissue-specific expression response to copper stress. We demonstrated that higher copper concentrations specifically affected MT3 expression among ecotypes. Our analysis shows that MT3a has similar expression pattern in cupricolous ecotypes while MT3b has common expression features shared by all metallophyte S. vulgaris ecotypes. Our data indicate that down-regulation of MT3b root expression in higher copper concentrations is associated with copper stress. We propose that there might be a specific regulation of SvMT3s transcription depending on the type of heavy metal tolerance.
See more in PubMed
Mol Gen Genet. 1995 Aug 21;248(3):318-28 PubMed
J Exp Bot. 2006;57(14):3575-82 PubMed
Curr Opin Plant Biol. 2011 Jun;14(3):252-9 PubMed
Ecotoxicology. 2012 Oct;21(7):2004-11 PubMed
Planta. 1984 Sep;162(2):174-9 PubMed
Plant Physiol. 2001 Aug;126(4):1519-26 PubMed
Plant Biol (Stuttg). 2011 Mar;13(2):225-32 PubMed
Genome Res. 2004 Jun;14(6):1147-59 PubMed
J Biol Inorg Chem. 2011 Oct;16(7):1035-45 PubMed
BMC Genomics. 2011 Jul 26;12:376 PubMed
J Plant Physiol. 2009 Mar 15;166(5):521-30 PubMed
Cell Biochem Biophys. 1999;31(1):19-48 PubMed
J Exp Bot. 2009;60(1):187-96 PubMed
PLoS One. 2012;7(2):e31898 PubMed
Planta. 2005 Nov;222(4):716-29 PubMed
Curr Opin Biotechnol. 2005 Apr;16(2):133-41 PubMed
J Exp Bot. 2004 Dec;55(408):2483-93 PubMed
Evolution. 1996 Oct;50(5):1888-1895 PubMed
FEBS Lett. 2004 Nov 5;577(1-2):9-16 PubMed
Science. 1985 May 10;228(4700):685-90 PubMed
Plant Mol Biol. 2004 Apr;54(6):805-15 PubMed
Plant Physiol. 2008 Apr;146(4):1697-706 PubMed
Methods. 2001 Dec;25(4):402-8 PubMed
Biotechniques. 2000 Jun;28(6):1102, 1104 PubMed
Environ Toxicol. 2010 Dec;25(6):581-92 PubMed
New Phytol. 2009 Mar;181(4):759-776 PubMed
Annu Rev Plant Biol. 2002;53:159-82 PubMed
J Mol Biol. 1990 Oct 5;215(3):403-10 PubMed
GENBANK
JX648329, JX648330, JX648331, JX648332, JX648333
