Immobilized synthetic pathway for biodegradation of toxic recalcitrant pollutant 1,2,3-trichloropropane
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24787668
DOI
10.1021/es500396r
Knihovny.cz E-zdroje
- MeSH
- Agrobacterium enzymologie MeSH
- biodegradace účinky léků MeSH
- biokatalýza účinky léků MeSH
- bioreaktory mikrobiologie MeSH
- biotransformace účinky léků MeSH
- časové faktory MeSH
- chemické látky znečišťující vodu metabolismus toxicita MeSH
- enzymy imobilizované metabolismus MeSH
- hydrolasy metabolismus MeSH
- metabolické sítě a dráhy * účinky léků MeSH
- propan analogy a deriváty chemie metabolismus toxicita MeSH
- Rhodococcus enzymologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,2,3-trichloropropane MeSH Prohlížeč
- chemické látky znečišťující vodu MeSH
- enzymy imobilizované MeSH
- haloalcohol dehalogenase MeSH Prohlížeč
- haloalkane dehalogenase MeSH Prohlížeč
- hydrolasy MeSH
- propan MeSH
The anthropogenic compound 1,2,3-trichloropropane (TCP) has recently drawn attention as an emerging groundwater contaminant. No living organism, natural or engineered, is capable of the efficient aerobic utilization of this toxic industrial waste product. We describe a novel biotechnology for transforming TCP based on an immobilized synthetic pathway. The pathway is composed of three enzymes from two different microorganisms: engineered haloalkane dehalogenase from Rhodococcus rhodochrous NCIMB 13064, and haloalcohol dehalogenase and epoxide hydrolase from Agrobacterium radiobacter AD1. Together, they catalyze consecutive reactions converting toxic TCP to harmless glycerol. The pathway was immobilized in the form of purified enzymes or cell-free extracts, and its performance was tested in batch and continuous systems. Using a packed bed reactor filled with the immobilized biocatalysts, 52.6 mmol of TCP was continuously converted into glycerol within 2.5 months of operation. The efficiency of the TCP conversion to the intermediates was 97%, and the efficiency of conversion to the final product glycerol was 78% during the operational period. Immobilized biocatalysts are suitable for removing TCP from contaminated water up to a 10 mM solubility limit, which is an order of magnitude higher than the concentration tolerated by living microorganisms.
Citace poskytuje Crossref.org
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