Immobilized synthetic pathway for biodegradation of toxic recalcitrant pollutant 1,2,3-trichloropropane
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24787668
DOI
10.1021/es500396r
Knihovny.cz E-resources
- MeSH
- Agrobacterium enzymology MeSH
- Biodegradation, Environmental drug effects MeSH
- Biocatalysis drug effects MeSH
- Bioreactors microbiology MeSH
- Biotransformation drug effects MeSH
- Time Factors MeSH
- Water Pollutants, Chemical metabolism toxicity MeSH
- Enzymes, Immobilized metabolism MeSH
- Hydrolases metabolism MeSH
- Metabolic Networks and Pathways * drug effects MeSH
- Propane analogs & derivatives chemistry metabolism toxicity MeSH
- Rhodococcus enzymology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2,3-trichloropropane MeSH Browser
- Water Pollutants, Chemical MeSH
- Enzymes, Immobilized MeSH
- haloalcohol dehalogenase MeSH Browser
- haloalkane dehalogenase MeSH Browser
- Hydrolases MeSH
- Propane MeSH
The anthropogenic compound 1,2,3-trichloropropane (TCP) has recently drawn attention as an emerging groundwater contaminant. No living organism, natural or engineered, is capable of the efficient aerobic utilization of this toxic industrial waste product. We describe a novel biotechnology for transforming TCP based on an immobilized synthetic pathway. The pathway is composed of three enzymes from two different microorganisms: engineered haloalkane dehalogenase from Rhodococcus rhodochrous NCIMB 13064, and haloalcohol dehalogenase and epoxide hydrolase from Agrobacterium radiobacter AD1. Together, they catalyze consecutive reactions converting toxic TCP to harmless glycerol. The pathway was immobilized in the form of purified enzymes or cell-free extracts, and its performance was tested in batch and continuous systems. Using a packed bed reactor filled with the immobilized biocatalysts, 52.6 mmol of TCP was continuously converted into glycerol within 2.5 months of operation. The efficiency of the TCP conversion to the intermediates was 97%, and the efficiency of conversion to the final product glycerol was 78% during the operational period. Immobilized biocatalysts are suitable for removing TCP from contaminated water up to a 10 mM solubility limit, which is an order of magnitude higher than the concentration tolerated by living microorganisms.
References provided by Crossref.org
Building the SynBio community in the Czech Republic from the bottom up: You get what you give
An Ultrasensitive Fluorescence Assay for the Detection of Halides and Enzymatic Dehalogenation
Detection of Chloroalkanes by Surface-Enhanced Raman Spectroscopy in Microfluidic Chips
Computational Study of Protein-Ligand Unbinding for Enzyme Engineering
A Pseudomonas putida strain genetically engineered for 1,2,3-trichloropropane bioremediation
