Detection of carbapenemases in Enterobacteriaceae: a challenge for diagnostic microbiological laboratories
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Review
PubMed
24813781
DOI
10.1111/1469-0691.12678
PII: S1198-743X(14)65088-6
Knihovny.cz E-resources
- Keywords
- Carba NP, Enterobacteriaceae, MALDI-TOF MS, carbapenemase, detection, β-Lactamase,
- MeSH
- Bacterial Proteins analysis genetics MeSH
- Bacteriological Techniques methods MeSH
- beta-Lactamases analysis genetics MeSH
- Molecular Diagnostic Techniques methods MeSH
- Enterobacteriaceae enzymology genetics MeSH
- Humans MeSH
- Mass Screening methods MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
- Names of Substances
- Bacterial Proteins MeSH
- beta-Lactamases MeSH
- carbapenemase MeSH Browser
Carbapenemase-producing bacteria have now spread all over the world. Infections caused by those bacteria are difficult to treat. Therefore, there is an urgent need for accurate and fast detection of carbapenemases in diagnostic laboratories. In this review, we summarize screening methods for suspected isolates, direct assays for confirmation of carbapenemase activity (e.g. the Carba NP test and matrix-assisted laser desorption ionization time-of-flight mass spectrometry carbapenem hydrolysis assay), inhibitor-based methods for carbapenemase classification, and molecular-genetic techniques for precise identification of carbapenemase genes. We also propose a workflow for carbapenemase identification in diagnostic laboratories.
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