Dermatophagoides farinae fecal allergens are a major source of immunogens in home environments; however, as the source of mite fecal allergen is considered spent growth medium extract that can only mimic the pure fecal extract. In this study, we prepared and using proteomic methods analyzed a D. farinae fecal extract for the first time. The preparation approach used D. farinae feces that were produced within 8 weeks of initiating cultivation in minimized growth media. The feces were collected via adhesion to the tissue culture flask surfaces after removing the SGM and mites. This study contains in-depth proteomic mapping of the allergenic isoforms from the D. farinae fecal extract. Despite extensive analysis, MALDI TOF/TOF spectrometry showed that only six proteins/allergens, Der f1, Der f2, Der f3, Der f6, Der f15 and ferritin, originated from D. farinae. No other analyzed proteins were exactly assigned to Dermatophagoides or to similar invertebrate species by sequence similarity. The remaining proteins were assigned mostly to yeasts or cereals (originally dietary proteins); however, many of the proteins were not successfully identified in the current NCBInr. The numerous dietary proteins identified in the feces suggest that these proteins remained highly stable after passing through the gut. Isoforms of the allergens Der f1, Der f3 and Der f15 were identified in more MWs indicating the presence of zymogens and active-enzyme forms. The identified fecal allergens accumulate in the environment during the life of the mite and represent quantitatively greater amounts of mite immunogens than those that were missed in the 2D-E. The results contribute to our understanding of D. farinae digestive physiology with regard to the enzymes/proteins present in the feces.

Biologically Active Substances in Crop Protection Laboratory of Proteomics Crop Research Institute Drnovska 507 73 16106 Praha 6 Ruzyne Czech Republic

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J Allergy Clin Immunol. 1998 Aug;102(2):295-303 PubMed

Allergy. 2002 Aug;57(8):729-31 PubMed

J Mol Biol. 2009 Feb 20;386(2):520-30 PubMed

Chang Gung Med J. 2004 Aug;27(8):563-9 PubMed

Clin Exp Allergy. 2002 Sep;32(9):1341-7 PubMed

J Allergy Clin Immunol. 2003 Jul;112(1):79-86 PubMed

J Med Entomol. 1997 Nov;34(6):684-9 PubMed

J Med Entomol. 1998 Nov;35(6):962-6 PubMed

J Med Entomol. 1996 Mar;33(2):257-60 PubMed

Int Arch Allergy Appl Immunol. 1985;76(1):58-67 PubMed

J Investig Allergol Clin Immunol. 1998 Jul-Aug;8(4):201-6 PubMed

Clin Exp Allergy. 1995 Aug;25(8):765-70 PubMed

Mol Cell Proteomics. 2013 Jul;12(7):1818-28 PubMed

J Allergy Clin Immunol. 2007 Jul;120(1):105-12 PubMed

Med Vet Entomol. 2011 Jun;25(2):209-16 PubMed

Allergy. 2004 Jun;59(6):571-4 PubMed

Int Arch Allergy Appl Immunol. 1991;95(2-3):248-56 PubMed

Arch Insect Biochem Physiol. 2010 Nov;75(3):187-206 PubMed

Immunology. 1992 Jan;75(1):29-35 PubMed

J Allergy Clin Immunol. 1989 Jun;83(6):1055-67 PubMed

Trends Mol Med. 2010 Jul;16(7):321-8 PubMed

Clin Exp Allergy. 2000 Sep;30(9):1293-7 PubMed

Allergy. 2010 Feb;65(2):184-90 PubMed

J Immunol. 2005 Sep 15;175(6):3835-45 PubMed

Arch Insect Biochem Physiol. 2009 Jul;71(3):139-58 PubMed

Clin Exp Allergy. 2005 Oct;35(10):1384-91 PubMed

Vet Immunol Immunopathol. 2001 Feb 10;78(3-4):231-47 PubMed

Nature. 1981 Feb 12;289(5798):592-3 PubMed

J Insect Sci. 2010;10:42 PubMed

Int Arch Allergy Immunol. 2006;140(4):295-305 PubMed

Exp Appl Acarol. 2008 Mar;44(3):199-212 PubMed

J Allergy Clin Immunol. 2006 Mar;117(3):571-6 PubMed

Immunol Rev. 2011 Jul;242(1):51-68 PubMed

Allergy. 2008 Jun;63(6):758-67 PubMed

J Immunol. 2013 Apr 1;190(7):3059-67 PubMed

J Med Entomol. 2010 Nov;47(6):1062-70 PubMed

J Allergy Clin Immunol. 1998 Oct;102(4 Pt 1):631-6 PubMed

Biochem Biophys Res Commun. 2005 Mar 25;328(4):944-52 PubMed

Exp Appl Acarol. 1992 Nov;16(1-2):165-80 PubMed

J Allergy Clin Immunol. 1989 Feb;83(2 Pt 1):416-27 PubMed

Int Arch Allergy Immunol. 2002 Sep;129(1):1-18 PubMed

J Med Entomol. 1991 Jul;28(4):487-91 PubMed

Ann Allergy Asthma Immunol. 2002 Jul;89(1):34-7 PubMed

J Allergy Clin Immunol. 1996 Oct;98(4):739-47 PubMed

Allergol Immunopathol (Madr). 1997 May-Jun;25(3):113-7 PubMed

Allergy Asthma Immunol Res. 2012 Nov;4(6):346-50 PubMed

Clin Exp Allergy. 1997 Feb;27(2):201-7 PubMed

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