Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

. 2014 ; 9 () : 3687-706. [epub] 20140806

Jazyk angličtina Země Nový Zéland Médium electronic-ecollection

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid25125978

Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0-N25 or M0-M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%-15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%-15% (v/v) nano-size or micro-size HA seems to be optimum.

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Rey C, Miquel JL, Facchini L, Legrand AP, Glimcher MJ. Hydroxyl groups in bone minerals. Bone. 1995;16(5):583–586. PubMed

Fratzl P, Gupta HS, Paschalis EP, Roschger P. Structure and mechanical quality of the collagen-mineral nano-composite in bone. J Mater Chem. 2004;14:2115–2123.

Cazalbou S, Eichert D, Ranz X, et al. Ion exchanges in apatites for biomedical application. J Mater Sci Mater Med. 2005;16(5):405–409. PubMed

Uskoković V, Uskoković DP. Nanosized hydroxyapatite and other calcium phosphates: Chemistry of formation and application as drug and gene delivery agents. J Biomed Mater Res B Appl Biomater. 2011;96(1):152–191. PubMed

LeGeros RZ. Calcium phosphate-based osteoinductive materials. Chem Soc Rev. 2008;108(11):4742–4753. PubMed

Ignjatovic NL, Ajdukovic ZR, Savic VP, Uskokovic DP. Size effect of calcium phosphate coated with poly-DL-lactide-co-glycolide on healing processes in bone reconstruction. J Biomed Mater Res B Appl Biomater. 2010;94(1):108–117. PubMed

Rubin MA, Jasiuk I, Taylor J, Rubin J, Ganey T, Apkarian RP. TEM analysis of the nanostructure of normal and osteoporotic human trabecular bone. Bone. 2003;33(3):270–282. PubMed

Shi X, Wang Y, Ren L, Zhao N, Gong Y, Wang DA. Novel mesoporous silica-based antibiotic releasing scaffold for bone repair. Acta Biomater. 2009;5(5):1697–1707. PubMed

Suarez-Gonzales D, Barnhart K, Saito E, Vanderby R, Jr, Hollister SJ, Murphy WL. Controlled nucleation of hydroxyapatite on alginate scaffolds for stem cell-based bone tissue engineering. J Biomed Mater Res A. 2010;95(1):222–234. PubMed PMC

Roohani-Esfahani S, Nouri-Khorasani S, Lu Z, Appleyard R, Zreiqat H. The influence hydroxyapatite shape and size on biphasic calcium phosphate scaffolds coated with hydroxyapatite-PCL composites. Biomaterials. 2010;31(21):5498–5509. PubMed

Miao X, Tan DM, Li J, Xiao Y, Crawford R. Mechanical and biological properties of hydroxyapatite/tricalcium phosphate scaffolds coated with poly(lactic-co-glycolic acid) Acta Biomater. 2008;4(3):638–645. PubMed

Matsuo A, Chiba H, Takahashi H, Toyoda J, Abukawa H. Clinical application of a custom-made bioresorbable raw particulate hydroxyapatite-poly-L-lactide mesh tray for mandibular reconstruction. Odontology. 2010;98(1):85–88. PubMed

Itokawa H, Hiraide T, Moriya M, et al. A 12 month in vivo study on the response of bone to a hydroxyapatite-polymethylmethacrylate cranioplasty composite. Biomaterials. 2007;28(33):4922–4927. PubMed

Tungtasana H, Shuangshoti S, Shuangshoti S, et al. Tissue response and biodegradation of composite scaffolds prepared from Thai silk fibroin, gelatin and hydroxyapatite. J Mater Sci Mater Med. 2010;21(12):3151–3162. PubMed

Chung EJ, Qiu H, Kodali P, et al. Early tissue response to citric acid-based micro- and nanocomposite. J Biomed Mater Res. 2011;96(1):29–37. PubMed PMC

Takeuchi A, Ohtsuki C, Kamitakahara M, Ogata S, Miyazaki T, Tanihara M. Biomimetic deposition of hydroxyapatite on synthetic polypeptide with β-sheet structure in a solution mimicking body fluid. J Mater Sci Mater Med. 2008;19(1):387–393. PubMed

Suchý T, Rýglová Š, Balík K, et al. Biological evaluation of polydimethylsiloxane modified by calcium phosphate nanoparticles for potential application in spine surgery. Sci Advan Mat. 2013;5(5):484–493.

Balik K, Suchy T, Sucharda Z, Ryglova Š, Denk F. Design for a filler of an intervertebral cage for spine treatment on the basis of fibers and particulate composites. Ceram Silik. 2009;53(4):310–314.

Jeon K, Oh HJ, Lima H, et al. Self-renewal of embryonic stem cells through culture on nanopattern polydimethylsiloxane substrate. Biomaterials. 2012;33:5206–5220. PubMed

Mussard W, Kebir N, Kriegel I, Esteve M, Semetey V. Facile and efficient control of bioadhesion on poly(dimethylsiloxane) by using a biomimetic approach. Angew Chem Int Ed Engl. 2011;50(46):10871–10874. PubMed

Abbasi F, Mirzadeh H, Karban A. Modification of polysiloxane polymers for biomedical applications: a review. Polym Int. 2001;50(12):1279–1287.

Balik K, Suchy T, Sucharda Z, et al. Effect of nano/micro particles of calcium phosphates on the mechanical properties of composites based on polysiloxane matrix reinforced by polyamide. Ceram Silik. 2008;52(4):260–267.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193(1):265–275. PubMed

Rodriguez-Rodriguez R, Muñoz-Berbel X, Demming S, Büttgenbach S, Herrera MD, Llobera A. Cell-based microfluidic device for screening anti-proliferative activity of drugs in vascular smooth muscle cells. Biomed Microdevices. 2012;14(6):1129–1140. PubMed

Vagaská B, Bacáková L, Filová E, Balík K. Osteogenic cells on bio-inspired materials for bone tissue engineering. Physiol Res. 2010;59(3):309–322. PubMed

Duan B, Wang M, Zhou WY, Cheung WL, Li ZY, Lu WW. Three-dimensional nanocomposite scaffolds fabricated via selective laser sintering for bone tissue engineering. Acta Biomater. 2010;6(12):4495–4505. PubMed

Dyke JC, Knight KJ, Zhou H, Chiu CK, Ko CC, You W. An investigation of siloxane cross-linked hydroxyapatite-gelatin/copolymer composites for potential orthopedic applications. J Mater Chem. 2012;22(43):22888–22898. PubMed PMC

Hou R, Zhang G, Du G, et al. Magnetic nanohydroxyapatite/PVA composite hydrogels for promoted osteoblast adhesion and proliferation. Colloids Surf B Biointerfaces. 2012;103C:318–325. PubMed

Gloria A, Russo T, D’Amora U, et al. Magnetic poly(ε-caprolactone)/iron-doped hydroxyapatite nanocomposite substrates for advanced bone tissue engineering. J R Soc Interface. 2013;10(80):20120833. PubMed PMC

Peng F, Yu X, Wei M. In vitro cell performance on hydroxyapatite particles/poly(L-lactic acid) nanofibrous scaffolds with an excellent particle along nanofiber orientation. Acta Biomater. 2011;7(6):2585–2592. PubMed

Heo SJ, Kim SE, Wei J, et al. Fabrication and characterization of novel nano- and micro-HA/PCL composite scaffolds using a modified rapid prototyping process. J Biomed Mater Res A. 2009;89(1):108–116. PubMed

Heo SJ, Kim SE, Wei J, et al. In vitro and animal study of novel nano-hydroxyapatite/poly(epsilon-caprolactone) composite scaffolds fabricated by layer manufacturing process. Tissue Eng Part A. 2009;15(5):977–989. PubMed

Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R. Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics. J Biomed Mater Res. 2000;51(3):475–483. PubMed

Bačáková L, Filová E, Pařízek M, Ruml T, Švorčík V. Modulation of cell adhesion, proliferation and differentiation on materials designed for body implants. Biotechnol Adv. 2011;29(6):739–767. PubMed

Huang Y, Zhou G, Zheng L, Liu H, Niu X, Fan Y. Micro-/nano- sized hydroxyapatite directs differentiation of rat bone marrow derived mesenchymal stem cells towards an osteoblast lineage. Nanoscale. 2012;4(7):2484–2490. PubMed

Anselme K. Osteoblast adhesion on biomaterials. Biomaterials. 2000;21(7):667–681. PubMed

Polini A, Pisignano D, Parodi M, Quarto R, Scaglione S. Osteoinduction of human mesenchymal stem cells by bioactive composite scaffolds without supplemental osteogenic growth factors. PLoS One. 2011;6:e26211–e26222. PubMed PMC

Barradas AM, Monticone V, Hulsman M, et al. Molecular mechanisms of biomaterial-driven osteogenic differentiation in human mesenchymal stromal cells. Integr Biol (Camb) 2013;5(7):920–931. PubMed

Xia L, Zhang Z, Chen L, et al. Proliferation and osteogenic differentiation of human periodontal ligament cells on akermanite and β-TCP bioceramics. Eur Cell Mater. 2011;22:68–82. PubMed

Gu H, Guo F, Zhou X, et al. The stimulation of osteogenic differentiation of human adipose-derived stem cells by ionic products from akermanite dissolution via activation of the ERK pathway. Biomaterials. 2011;32(29):7023–7033. PubMed

An S, Gao Y, Ling J, Wei X, Xiao Y. Calcium ions promote osteogenic differentiation and mineralization of human dental pulp cells: implications for pulp capping materials. J Mater Sci Mater Med. 2012;23(3):789–795. PubMed

Qing F, Wang Z, Hong Y, et al. Selective effects of hydroxyapatite nanoparticles on osteosarcoma cells and osteoblasts. J Mater Sci Mater Med. 2012;23(9):2245–2251. PubMed

Kumar A, Dhara S, Biswas K, Basu B. In vitro bioactivity and cytocompatibility properties of spark plasma sintered HA-Ti composites. J Biomed Mater Res B Appl Biomater. 2013;101(2):223–236. PubMed

Hoppe A, Güldal NS, Boccaccini AR. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials. 2011;32(11):2757–2774. PubMed

Maeno S, Niki Y, Matsumoto H, et al. The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials. 2005;26(23):4847–4855. PubMed

Shi Z, Huang X, Liu B, Tao H, Cai Y, Tang R. Biological response of osteosarcoma cells to size-controlled nanostructured hydroxyapatite. J Biomater Appl. 2010;25(1):19–37. PubMed

Xu JL, Khor KA, Sui JJ, Zhang JH, Chen WN. Protein expression profiles in osteoblasts in response to differentially shaped hydroxyapatite nanoparticles. Biomaterials. 2009;30(29):5385–5391. PubMed

Xiong J, Li Y, Hodgson PD, Wen C. In vitro osteoblast-like cell proliferation on nano-hydroxyapatite coatings with different morphologies on a titanium-niobium shape memory alloy. J Biomed Mater Res A. 2010;95(3):766–773. PubMed

Ramakrishna S, Mayer J, Wintermantel E, Leong KW. Biomedical applications of polymer-composite materials: a review. Comp Sci Tech. 2001;61(1):1189–1224.

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