Plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in Hypoxis hemerocallidea organ and callus cultures
Language English Country Ireland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25219317
DOI
10.1016/j.plantsci.2014.08.003
PII: S0168-9452(14)00188-5
Knihovny.cz E-resources
- Keywords
- Antioxidants, Hydroxybenzoic acids, Hydroxycinnamic acids, Hypoxidaceae, Organogenesis, Secondary metabolites,
- MeSH
- Antioxidants metabolism pharmacology MeSH
- Biphenyl Compounds metabolism MeSH
- Cytokinins pharmacology MeSH
- Phenols metabolism pharmacology MeSH
- Hydroxybenzoates metabolism pharmacology MeSH
- Hypoxis growth & development metabolism MeSH
- Isopentenyladenosine pharmacology MeSH
- Coumaric Acids metabolism pharmacology MeSH
- Picrates metabolism MeSH
- Regeneration MeSH
- Plant Growth Regulators pharmacology MeSH
- Plant Somatic Embryogenesis Techniques MeSH
- Plant Structures growth & development metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,1-diphenyl-2-picrylhydrazyl MeSH Browser
- Antioxidants MeSH
- Biphenyl Compounds MeSH
- Cytokinins MeSH
- Phenols MeSH
- Hydroxybenzoates MeSH
- Isopentenyladenosine MeSH
- Coumaric Acids MeSH
- N(6)-(delta(2)-isopentenyl)adenine MeSH Browser
- Picrates MeSH
- Plant Growth Regulators MeSH
Micropropagation of Hypoxis hemerocallidea Fisch. and C.A. Mey was used as a model system to study the influence of cytokinins (CKs) on plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in organ and callus cultures and their antioxidant activity. Fourteen free phenolic acids were detected using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) while antioxidant activity was evaluated using oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity. Cytokinins had a significant effect on the biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in H. hemerocallidea organ cultures. In particular, meta-topolin-treated organ cultures produced high concentrations of gallic, protocatechuic, gentisic, p-hydroxybenzoic, m-hydroxybenzoic, salicylic, chlorogenic and trans-cinnamic acids. The isoprenoid CK, N(6)-(2-isopentenyl)-adenine significantly increased the accumulation of hydroxycinnamic acid derivatives, namely, caffeic, p-coumaric, sinapic and ferulic acids. Cytokinin-treated organ cultures exhibited a significant increase in antioxidant activity, particularly in the ORAC model. In callus cultures, CKs decreased the concentrations of hydroxycinnamic acid derivatives and antioxidant activity when compared to the control. Overall, both CK type and concentration had a significant effect on plant regeneration, callus proliferation, biochemical accumulation of free phenolic acids and antioxidant activity of the resultant extracts.
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