Plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in Hypoxis hemerocallidea organ and callus cultures
Jazyk angličtina Země Irsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25219317
DOI
10.1016/j.plantsci.2014.08.003
PII: S0168-9452(14)00188-5
Knihovny.cz E-zdroje
- Klíčová slova
- Antioxidants, Hydroxybenzoic acids, Hydroxycinnamic acids, Hypoxidaceae, Organogenesis, Secondary metabolites,
- MeSH
- antioxidancia metabolismus farmakologie MeSH
- bifenylové sloučeniny metabolismus MeSH
- cytokininy farmakologie MeSH
- fenoly metabolismus farmakologie MeSH
- hydroxybenzoáty metabolismus farmakologie MeSH
- Hypoxis růst a vývoj metabolismus MeSH
- isopentenyladenosin farmakologie MeSH
- kyseliny kumarové metabolismus farmakologie MeSH
- pikráty metabolismus MeSH
- regenerace MeSH
- regulátory růstu rostlin farmakologie MeSH
- somatická embryogeneze rostlin MeSH
- stavba rostlin růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1,1-diphenyl-2-picrylhydrazyl MeSH Prohlížeč
- antioxidancia MeSH
- bifenylové sloučeniny MeSH
- cytokininy MeSH
- fenoly MeSH
- hydroxybenzoáty MeSH
- isopentenyladenosin MeSH
- kyseliny kumarové MeSH
- N(6)-(delta(2)-isopentenyl)adenine MeSH Prohlížeč
- pikráty MeSH
- regulátory růstu rostlin MeSH
Micropropagation of Hypoxis hemerocallidea Fisch. and C.A. Mey was used as a model system to study the influence of cytokinins (CKs) on plant regeneration and biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in organ and callus cultures and their antioxidant activity. Fourteen free phenolic acids were detected using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) while antioxidant activity was evaluated using oxygen radical absorbance capacity (ORAC) and 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity. Cytokinins had a significant effect on the biochemical accumulation of hydroxybenzoic and hydroxycinnamic acid derivatives in H. hemerocallidea organ cultures. In particular, meta-topolin-treated organ cultures produced high concentrations of gallic, protocatechuic, gentisic, p-hydroxybenzoic, m-hydroxybenzoic, salicylic, chlorogenic and trans-cinnamic acids. The isoprenoid CK, N(6)-(2-isopentenyl)-adenine significantly increased the accumulation of hydroxycinnamic acid derivatives, namely, caffeic, p-coumaric, sinapic and ferulic acids. Cytokinin-treated organ cultures exhibited a significant increase in antioxidant activity, particularly in the ORAC model. In callus cultures, CKs decreased the concentrations of hydroxycinnamic acid derivatives and antioxidant activity when compared to the control. Overall, both CK type and concentration had a significant effect on plant regeneration, callus proliferation, biochemical accumulation of free phenolic acids and antioxidant activity of the resultant extracts.
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