Synthesis and biological evaluation of nandrolone-bodipy conjugates
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25448362
DOI
10.1016/j.steroids.2014.10.002
PII: S0039-128X(14)00252-9
Knihovny.cz E-resources
- Keywords
- Androgens, Cytotoxicity, Fluorescent conjugates, Nandrolone,
- MeSH
- Fluorescent Dyes chemical synthesis chemistry MeSH
- Microscopy, Fluorescence MeSH
- HEK293 Cells MeSH
- HeLa Cells MeSH
- Humans MeSH
- MCF-7 Cells MeSH
- Molecular Conformation MeSH
- Cell Line, Tumor MeSH
- Nandrolone chemistry pharmacology MeSH
- Cell Proliferation drug effects MeSH
- Boron Compounds chemistry pharmacology MeSH
- Dose-Response Relationship, Drug MeSH
- Structure-Activity Relationship MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene MeSH Browser
- Fluorescent Dyes MeSH
- Nandrolone MeSH
- Boron Compounds MeSH
Here, we report synthesis and biological evaluation of fluorescent nandrolone-3-carboxymethyloxime derivatives conjugated with green-emitting bodipy dye via PEG linkers. All the newly-synthesized compounds were evaluated for their effect on cell proliferation in vitro in MCF-7, LNCaP, PC-3 and HEK 293T model cell lines using WST-1 assay. By means of live-cell fluorescence microscopy, the intracellular localization of nandrolone-bodipy conjugates was revealed in endoplasmic reticulum. Moreover, we performed competitive localization study with nonfluorescent nandrolone, metandrolone, boldenone, trenbolone, and testosterone.
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