Gut virome sequencing in children with early islet autoimmunity
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25678103
DOI
10.2337/dc14-2490
PII: dc14-2490
Knihovny.cz E-resources
- MeSH
- Autoimmunity immunology MeSH
- Autoantibodies metabolism MeSH
- Diabetes Mellitus, Type 1 immunology virology MeSH
- Child MeSH
- Feces virology MeSH
- Genotype MeSH
- Hyperglycemia immunology virology MeSH
- Infant MeSH
- Real-Time Polymerase Chain Reaction methods MeSH
- Islets of Langerhans immunology MeSH
- Humans MeSH
- Child, Preschool MeSH
- Intestines virology MeSH
- Case-Control Studies MeSH
- Viruses isolation & purification MeSH
- Check Tag
- Child MeSH
- Infant MeSH
- Humans MeSH
- Male MeSH
- Child, Preschool MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Autoantibodies MeSH
OBJECTIVE: This study used next-generation sequencing (NGS) technologies to characterize the gut virome at the onset of islet autoimmunity. RESEARCH DESIGN AND METHODS: We conducted a case-control study nested within the Finnish Diabetes Prediction and Prevention (DIPP) cohort. The stool virome in 19 case children, who turned islet autoantibody positive before the age of 2 years and later developed clinical type 1 diabetes, and 19 tightly matched control subjects was analyzed using NGS performed from stool samples collected 3, 6, and 9 months before the onset of islet autoimmunity. Human virus findings were verified using real-time PCR. RESULTS: One or more human viruses were present in 10.4% and bacteriophages were in 54% of the samples. The virome composition showed no association with islet autoimmunity. NGS was less sensitive and specific than real-time PCR. CONCLUSIONS: The present data suggest no dramatic changes in the gut virome shortly before the emergence of islet autoimmunity and emphasize the need of verification of mass sequencing results when viral exposure is assessed in association studies.
Department of Immunogenetics University of Turku Turku Finland
Department of Medical Biochemistry and Genetics University of Turku Turku Finland
Department of Pediatrics Oulu University Hospital and University of Oulu Oulu Finland
Department of Pediatrics Turku University Central Hospital Turku Finland
Department of Virology School of Medicine University of Tampere Tampere Finland
References provided by Crossref.org