OBJECTIVE: This study used next-generation sequencing (NGS) technologies to characterize the gut virome at the onset of islet autoimmunity. RESEARCH DESIGN AND METHODS: We conducted a case-control study nested within the Finnish Diabetes Prediction and Prevention (DIPP) cohort. The stool virome in 19 case children, who turned islet autoantibody positive before the age of 2 years and later developed clinical type 1 diabetes, and 19 tightly matched control subjects was analyzed using NGS performed from stool samples collected 3, 6, and 9 months before the onset of islet autoimmunity. Human virus findings were verified using real-time PCR. RESULTS: One or more human viruses were present in 10.4% and bacteriophages were in 54% of the samples. The virome composition showed no association with islet autoimmunity. NGS was less sensitive and specific than real-time PCR. CONCLUSIONS: The present data suggest no dramatic changes in the gut virome shortly before the emergence of islet autoimmunity and emphasize the need of verification of mass sequencing results when viral exposure is assessed in association studies.

• MeSH
• autoimunita imunologie   MeSH
• autoprotilátky metabolismus   MeSH
• diabetes mellitus 1. typu imunologie   virologie   MeSH
• dítě MeSH
• feces virologie   MeSH
• genotyp MeSH
• hyperglykemie imunologie   virologie   MeSH
• kojenec MeSH
• kvantitativní polymerázová řetězová reakce metody   MeSH
• Langerhansovy ostrůvky imunologie   MeSH
• lidé MeSH
• předškolní dítě MeSH
• střeva virologie   MeSH
• studie případů a kontrol MeSH
• viry izolace a purifikace   MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• lidé MeSH
• mužské pohlaví MeSH
• předškolní dítě MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH