3D-printed biosensor with poly(dimethylsiloxane) reservoir for magnetic separation and quantum dots-based immunolabeling of metallothionein
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Biosensor, Bioseparation, Head and neck cancer, Metallothionein, Nanotechnology,
- MeSH
- Printing, Three-Dimensional * MeSH
- Biosensing Techniques MeSH
- Dimethylpolysiloxanes chemistry MeSH
- Fluorescence MeSH
- Metal Nanoparticles MeSH
- Quantum Dots MeSH
- Humans MeSH
- Magnetics MeSH
- Metallothionein analysis MeSH
- Cell Line, Tumor MeSH
- Neoplasms pathology MeSH
- Cadmium Compounds chemistry MeSH
- Tellurium chemistry MeSH
- Gold chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- baysilon MeSH Browser
- cadmium telluride MeSH Browser
- Dimethylpolysiloxanes MeSH
- Metallothionein MeSH
- Cadmium Compounds MeSH
- Tellurium MeSH
- Gold MeSH
Currently, metallothioneins (MTs) are extensively investigated as the molecular biomarkers and the significant positive association of the MT amount was observed in tumorous versus healthy tissue of various types of malignant tumors, including head and neck cancer. Thus, we proposed a biosensor with fluorescence detection, comprising paramagnetic nanoparticles (nanomaghemite core with gold nanoparticles containing shell) for the magnetic separation of MT, based on affinity of its sulfhydryl groups toward gold. Biosensor was crafted from PDMS combined with technology of 3D printing and contained reservoir with volume of 50 μL linked to input (sample/detection components and washing/immunobuffer) and output (waste). For the immunolabeling of immobilized MT anti-MT antibodies conjugated to CdTe quantum dots through synthetic heptapeptide were employed. After optimization of fundamental conditions of the immunolabeling (120 min, 20°C, and 1250 rpm) we performed it on a surface of paramagnetic nanoparticles in the biosensor reservoir, with evaluation of fluorescence of quantum dots (λexc 400 nm, and λem 555 nm). The developed biosensor was applied for quantification of MT in cell lines derived from spinocellular carcinoma (cell line 122P-N) and fibroblasts (122P-F) and levels of the biomarker were found to be about 90 nM in tumor cells and 37 nM in fibroblasts. The proposed system is able to work with low volumes (< 100 μL), with low acquisition costs and high portability.
Central European Institute of Technology Brno University of Technology Brno Czech Republic
Department of Biochemistry Faculty of Science Charles University Prague Prague Czech Republic
Department of Chemistry and Biochemistry Mendel University Brno Czech Republic
Department of Pathological Physiology Faculty of Medicine Masaryk University Brno Czech Republic
References provided by Crossref.org
Magnetic Nanoparticles: From Design and Synthesis to Real World Applications
