The protective effect of pyrimidine nucleosides on human HaCaT keratinocytes treated with 5-FU
Jazyk angličtina Země Řecko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25750279
PII: 35/3/1303
Knihovny.cz E-zdroje
- Klíčová slova
- 5-FU, Hand-foot syndrome, cytidine, deoxycytidine, keratinocytes, thymidine, uridine,
- MeSH
- dihydrouracildehydrogenasa (NADP) fyziologie MeSH
- fluoruracil škodlivé účinky MeSH
- keratinocyty účinky léků MeSH
- kultivované buňky MeSH
- lidé MeSH
- protinádorové antimetabolity škodlivé účinky MeSH
- pyrimidinové nukleosidy farmakologie MeSH
- tetrazoliové soli MeSH
- thiazoly MeSH
- thymidylátsynthasa antagonisté a inhibitory MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- dihydrouracildehydrogenasa (NADP) MeSH
- fluoruracil MeSH
- protinádorové antimetabolity MeSH
- pyrimidinové nukleosidy MeSH
- tetrazoliové soli MeSH
- thiazoly MeSH
- thiazolyl blue MeSH Prohlížeč
- thymidylátsynthasa MeSH
BACKGROUND: Therapy with 5-fluorouracil (5-FU) and capecitabine is often complicated by skin toxicity (hand-foot syndrome, HFS). Topical application of uridine ointment is beneficial for alleviating HFS and other pyrimidine nucleosides have been described as 5-FU toxicity modulators. We tested pyrimidine nucleosides and their combinations to find the best combination for topical therapy of HFS. MATERIALS AND METHODS: Cellular viability was measured by the real-time cell analyser and methyl thiazol tetrazolium (MTT) assay in order to evaluate the effect of pyrimidine nucleosides on HaCaT keratinocytes treated with 5-FU. The results were confirmed by evaluation of the cellular colonization by microphotography. RESULTS: Cytidine and uridine protected keratinocytes to the same extent. Thymidine enhanced the protective effect when added to cytidine or uridine. Deoxycytidine did not have any protective effect. CONCLUSION: Our findings support the rationale for using uridine or cytidine in combination with thymidine in ointment for HFS treatment.
Central European Institute of Technology Brno University of Technology Brno Czech Republic
Prague Burn Centre 3rd Faculty of Medicine Charles University Prague Czech Republic
Synthesis and Anticancer and Antiviral Activities of C-2'-Branched Arabinonucleosides
