Onecut1 and Onecut2 transcription factors operate downstream of Pax6 to regulate horizontal cell development
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25794677
DOI
10.1016/j.ydbio.2015.02.023
PII: S0012-1606(15)00116-5
Knihovny.cz E-zdroje
- Klíčová slova
- Horizontal cell, Onecut, Pax6, Retina,
- MeSH
- čípky retiny metabolismus MeSH
- fenotyp MeSH
- hepatocytární jaderný faktor 6 metabolismus MeSH
- homeodoménové proteiny metabolismus MeSH
- hybridizace in situ MeSH
- kmenové buňky cytologie MeSH
- molekulární sekvence - údaje MeSH
- mutace MeSH
- myši transgenní MeSH
- myši MeSH
- oční proteiny metabolismus MeSH
- represorové proteiny metabolismus MeSH
- retina embryologie metabolismus MeSH
- sekvence nukleotidů MeSH
- sekvenční homologie nukleových kyselin MeSH
- transkripční faktor PAX6 MeSH
- transkripční faktory paired box metabolismus MeSH
- transkripční faktory metabolismus MeSH
- vazebná místa MeSH
- vývojová regulace genové exprese MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- hepatocytární jaderný faktor 6 MeSH
- homeodoménové proteiny MeSH
- oční proteiny MeSH
- Onecut1 protein, mouse MeSH Prohlížeč
- ONECUT2 protein, mouse MeSH Prohlížeč
- Pax6 protein, mouse MeSH Prohlížeč
- represorové proteiny MeSH
- transkripční faktor PAX6 MeSH
- transkripční faktory paired box MeSH
- transkripční faktory MeSH
Genetic studies of the last decades strongly indicated that generation of particular retinal cell types is governed by gene regulatory networks of transcription factors and their target genes. The paired and homeodomain transcription factor Pax6 plays a pivotal role in retinal development as its inactivation in the retinal progenitor cell population leads to abolished differentiation of all retinal cell types. However, until now, only a few transcription factors operating downstream of Pax6 responsible for generation of individual retinal cell types have been identified. In this study, we identified two transcription factors of the Onecut family, Onecut1 and Onecut2, as Pax6 downstream-acting factors. Onecut1 and Onecut2 were previously shown to be expressed in developing horizontal cells, retinal ganglion cells and cone photoreceptors; however, their role in differentiation of these cell types is poorly understood. In this study, we show that the horizontal cell genesis is severely disturbed in Onecut-deficient retinae. In single Onecut1 and Onecut2 mutants, the number of horizontal cells is dramatically reduced while horizontal cells are completely missing in the Onecut1/Onecut2 compound mutant. Analysis of genes involved in the horizontal cell genesis such as Foxn4, Ptf1a, Prox1 and Lim1 showed that although horizontal cells are initially formed, they are not maintained in Onecut-deficient retinae. Taken together, this study suggests the model in which Pax6 regulates the maintenance of horizontal cells through the activation of Onecut1 and Onecut2 transcription factors.
Citace poskytuje Crossref.org
Ancestral role of Pax6 in chordate brain regionalization
Dose-dependent regulation of horizontal cell fate by Onecut family of transcription factors
