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Spatiotemporal Dynamics of the BRI1 Receptor and its Regulation by Membrane Microdomains in Living Arabidopsis Cells

Wang, Li
Author Wang, Li Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Li, Hong
Author Li, Hong Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Lv, Xueqin
Author Lv, Xueqin Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
Chen, Tong
Author Chen, Tong Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Li, Ruili
Author Li, Ruili College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China
Xue, Yiqun
Author Xue, Yiqun Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Jiang, Jianjun
Author Jiang, Jianjun State Key Laboratory of Genetic Engineering and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China
Jin, Biao
Author Jin, Biao College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
Baluška, František
Author Baluška, František Institute of Cellular and Molecular Botany, University of Bonn, Kirschallee 1, D-53115 Bonn, Germany
Šamaj, Jozef
Author Šamaj, Jozef Department of Cell Biology, Palacky University Olomouc, Olomouc 78371, Czech Republic
Wang, Xuelu
Author Wang, Xuelu State Key Laboratory of Genetic Engineering and Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Lin, Jinxing
Author Lin, Jinxing Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: linjinxing@bjfu.edu.cn

Molecular plant. 2015 Sep ; 8 (9) : 1334-49. [epub] 20150418

Mol Plant
ISSN 1752-9867 | 1674-2052
Source

Language English Country Great Britain, England Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link https://www.medvik.cz/link/pmid25896454

PubMed 25896454
DOI 10.1016/j.molp.2015.04.005
PII: S1674-2052(15)00202-6
Knihovny.cz E-resources

Keywords
BR signaling, BRI1, endocytosis, membrane microdomains, spatiotemporal dynamics,
MeSH
Arabidopsis cytology metabolism MeSH
Brassinosteroids pharmacology MeSH
Spatio-Temporal Analysis * MeSH
Diffusion MeSH
Endocytosis drug effects MeSH
Clathrin metabolism MeSH
Membrane Microdomains drug effects metabolism MeSH
Protein Multimerization drug effects MeSH
Motion MeSH
Protein Kinases metabolism MeSH
Arabidopsis Proteins metabolism MeSH
Plant Cells drug effects metabolism MeSH
Signal Transduction drug effects MeSH
Publication type
Journal Article MeSH
Research Support, Non-U.S. Gov't MeSH
Names of Substances
Brassinosteroids MeSH
BRI1 protein, Arabidopsis MeSH Browser
Clathrin MeSH
Protein Kinases MeSH
Arabidopsis Proteins MeSH

The major brassinosteroid (BR) receptor of Arabidopsis BRASSINOSTEROID INSENSITIVE1 (BRI1) plays fundamental roles in BR signaling, but the molecular mechanisms underlying the effects of BR on BRI1 internalization and assembly state remain unclear. Here, we applied variable angle total internal reflection fluorescence microscopy and fluorescence cross-correlation spectroscopy to analyze the dynamics of GFP-tagged BRI1. We found that, in response to BR, the degree of co-localization of BRI1-GFP with AtFlot1-mCherry increased, and especially BR stimulated the membrane microdomain-associated pathway of BRI1 internalization. We also verified these observations in endocytosis-defective chc2-1 mutants and the AtFlot1 amiRNA 15-5 lines. Furthermore, examination of the phosphorylation status of bri1-EMS-suppressor 1 and measurement of BR-responsive gene expression revealed that membrane microdomains affect BR signaling. These results suggest that BR promotes the partitioning of BRI1 into functional membrane microdomains to activate BR signaling.

College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China

College of Horticulture and Plant Protection Yangzhou University Yangzhou 225009 China

Department of Cell Biology Palacky University Olomouc Olomouc 78371 Czech Republic

Institute of Cellular and Molecular Botany University of Bonn Kirschallee 1 D 53115 Bonn Germany

Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China

Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China; College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 China

Key Laboratory of Plant Resources Institute of Botany Chinese Academy of Sciences Beijing 100093 China; College of Horticulture and Plant Protection Yangzhou University Yangzhou 225009 China

State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China

State Key Laboratory of Genetic Engineering and Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China; College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China

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