Chitosan hydrogels enriched with polyphenols: Antibacterial activity, cell adhesion and growth and mineralization
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26050898
DOI
10.1016/j.carbpol.2015.04.043
PII: S0144-8617(15)00359-8
Knihovny.cz E-resources
- Keywords
- Antibacterial, Antioxidant, Cytocompatibility, Hydrogel, Polyphenol,
- MeSH
- Alkaline Phosphatase metabolism MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Cell Adhesion drug effects MeSH
- Cell Line MeSH
- Chitosan pharmacology MeSH
- Hydrogels pharmacology MeSH
- Kinetics MeSH
- Humans MeSH
- Microbial Sensitivity Tests MeSH
- Minerals chemistry MeSH
- Osteoblasts cytology drug effects MeSH
- Polyphenols pharmacology MeSH
- Cell Proliferation drug effects MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alkaline Phosphatase MeSH
- Anti-Bacterial Agents MeSH
- Chitosan MeSH
- Hydrogels MeSH
- Minerals MeSH
- Polyphenols MeSH
Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na-β-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1h, 3h and 6h incubation and slowed E. coli growth in liquid culture for 150min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth.
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