Reliable reference gene selection for quantitative real time PCR in Haemonchus contortus
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26255779
DOI
10.1016/j.molbiopara.2015.08.001
PII: S0166-6851(15)30019-0
Knihovny.cz E-resources
- Keywords
- Anthelmintic, Haemonchus contortus, Reference gene, qPCR,
- MeSH
- Anthelmintics pharmacology MeSH
- Haemonchus drug effects genetics MeSH
- Real-Time Polymerase Chain Reaction methods standards MeSH
- Reference Standards * MeSH
- Gene Expression Profiling methods standards MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Anthelmintics MeSH
The aim of this work was to identify reliable reference genes for expression studies in adult Haemonchus contortus. Eleven candidate genes were identified and the stability of their expression was assessed in adult males and females of two genetically divergent H. contortus isolates: drug-susceptible (ISE) and multi-drug-resistant (WR). Five genes with the most stable expression patterns were further assessed for suitability as reference genes in anthelmintic-treated H. contortus adults versus non-treated controls. We identified important differences in the expression of a number of candidate genes in anthelmintic-treated samples, confirming the need for careful validation of control genes for such experiments. We propose the use of multiple reference genes for expression studies in this species and found gpd, ama and far most suitable for adult H. contortus.
References provided by Crossref.org
UDP-Glycosyltransferases and Albendazole Metabolism in the Juvenile Stages of Haemonchus contortus