Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26380879
DOI
10.1021/acs.est.5b02049
Knihovny.cz E-zdroje
- MeSH
- buněčné linie účinky léků MeSH
- chemické látky znečišťující vodu toxicita MeSH
- imunologické faktory toxicita MeSH
- interleukin-6 metabolismus MeSH
- makrofágy účinky léků metabolismus patologie MeSH
- mikrocystiny toxicita MeSH
- mořské toxiny MeSH
- myši MeSH
- NF-kappa B metabolismus MeSH
- přirozená imunita účinky léků MeSH
- proteinfosfatasa 2 metabolismus MeSH
- sinice patogenita MeSH
- testy chronické toxicity metody MeSH
- TNF-alfa metabolismus MeSH
- zánět chemicky indukované imunologie metabolismus MeSH
- zásobování vodou MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chemické látky znečišťující vodu MeSH
- cyanoginosin LR MeSH Prohlížeč
- imunologické faktory MeSH
- interleukin-6, mouse MeSH Prohlížeč
- interleukin-6 MeSH
- mikrocystiny MeSH
- mořské toxiny MeSH
- NF-kappa B MeSH
- proteinfosfatasa 2 MeSH
- TNF-alfa MeSH
Microcystins (MCs) are primarily hepatotoxins produced by cyanobacteria and are responsible for intoxication in humans and animals. There are many incidents of chronic exposure to MCs, which have been attributed to the inappropriate treatment of water supplies or contaminated food. Using RAW 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-inflammatory cytokines (tumor necrosis factor α and interleukin-6) as a consequence of fast nuclear factor κB and nitrogen-activated protein kinase activation. In contrast to other studies, the observed effects were not attributed to the intracellular inhibition of protein phosphatases 1/2A due to lack of specific transmembrane transporters for MCs. However, the MC-LR-induced activation of macrophages was effectively inhibited by a specific peptide that blocks signaling of receptors, which play a pivotal role in the innate immune responses. Taken together, we showed for the first time that MC-LR could interfere with macrophage receptors that are responsible for triggering the above-mentioned signaling pathways. These findings provide an interesting mechanistic explanation of some adverse health outcomes associated with toxic cyanobacteria and MCs.
Faculty of Science RECETOX Masaryk University Kamenice 753 5 62500 Brno Czech Republic
Institute of Biophysics Academy of Sciences Královopolská 135 612 65 Brno Czech Republic
Citace poskytuje Crossref.org
