Novel method of simultaneous multiple immunogold localization on resin sections in high resolution scanning electron microscopy
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26472050
DOI
10.1016/j.nano.2015.09.008
PII: S1549-9634(15)00186-0
Knihovny.cz E-zdroje
- Klíčová slova
- BSE imaging, Gold nanoparticles, High resolution SEM, Multiple immunolabeling, STEM imaging,
- MeSH
- akrylové pryskyřice chemie MeSH
- barvení a značení metody MeSH
- imunohistochemie MeSH
- kovové nanočástice chemie ultrastruktura MeSH
- mikrotomie metody MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- skenovací elektrochemická mikroskopie metody MeSH
- vylepšení obrazu metody MeSH
- zlato chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- akrylové pryskyřice MeSH
- zlato MeSH
We present a new method of multiple immunolabeling that is suitable for a broad spectrum of biomedical applications. The general concept is to label both sides of the ultrathin section with the thickness of 70-80 nm with different antibodies conjugated to gold nanoparticles and to distinguish the labeled side by advanced imaging methods with high resolution scanning electron microscopy, such as by correlating images acquired at different energies of primary electrons using different signals. From the Clinical Editor: The use of transmission electron microscopy has become an indispensible tool in the detection of cellular proteins. In this short but interesting article, the authors described their new method of labeling and the identification of four different proteins simultaneously, which represents another advance in imaging technique.
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