Lipid-Based Diets Improve Muscarinic Neurotransmission in the Hippocampus of Transgenic APPswe/PS1dE9 Mice
Language English Country United Arab Emirates Media print
Document type Journal Article
PubMed
26502816
DOI
10.2174/1567205012666151027130350
PII: CAR-EPUB-71321
Knihovny.cz E-resources
- MeSH
- Alzheimer Disease diet therapy physiopathology MeSH
- Amyloid beta-Protein Precursor genetics metabolism MeSH
- Dietary Fats administration & dosage MeSH
- Hippocampus physiopathology MeSH
- Caspase 8 metabolism MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Mice, Inbred C3H MeSH
- Mice, Inbred C57BL MeSH
- Mice, Transgenic MeSH
- Synaptic Transmission physiology MeSH
- Presenilin-1 genetics metabolism MeSH
- Receptors, Muscarinic metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Amyloid beta-Protein Precursor MeSH
- APP protein, human MeSH Browser
- Casp8 protein, mouse MeSH Browser
- Dietary Fats MeSH
- Caspase 8 MeSH
- Presenilin-1 MeSH
- PSEN1 protein, human MeSH Browser
- Receptors, Muscarinic MeSH
Transgenic APPswe/PS1dE9 mice modeling Alzheimer's disease demonstrate ongoing accumulation of β-amyloid fragments resulting in formation of amyloid plaques that starts at the age of 4-5 months. Buildup of β-amyloid fragments is accompanied by impairment of muscarinic transmission that becomes detectable at this age, well before the appearance of cognitive deficits that manifest around the age of 12 months. We have recently demonstrated that long-term feeding of trangenic mice with specific isocaloric fish oil-based diets improves specific behavioral parameters. Now we report on the influence of short-term feeding (3 weeks) of three isocaloric diets supplemented with Fortasyn (containing fish oil and ingredients supporting membrane renewal), the plant sterol stigmasterol together with fish oil, and stigmasterol alone on markers of cholinergic neurotransmission in the hippocampus of 5-month-old transgenic mice and their wild-type littermates. Transgenic mice fed normal diet demostrated increase in ChAT activity and attenuation of carbachol-stimulated GTP-γ(35)S binding compared to wild-type mice. None of the tested diets compared to control diet influenced the activities of ChAT, AChE, BuChE, muscarinic receptor density or carbachol-stimulated GTP-γ(35)S binding in wild-type mice. In contrast, all experimental diets increased the potency of carbachol in stimulating GTP-γ(35)S binding in trangenic mice to the level found in wild-type animals. Only the Fortasyn diet increased markers of cholinergic synapses in transgenic mice. Our data demonstrate that even short-term feeding of transgenic mice with chow containing specific lipid-based dietary supplements can influence markers of cholinergic synapses and rectify impaired muscarinic signal transduction that develops in transgenic mice.
Institute of Physiology CAS Department of Neurochemistry Vídenska 1083 14220 Prague 4 Czech Republic
References provided by Crossref.org
Cholesterol as a key player in amyloid β-mediated toxicity in Alzheimer's disease
The Role of Lipid Environment in Ganglioside GM1-Induced Amyloid β Aggregation
Current Advances in Allosteric Modulation of Muscarinic Receptors
