Evaluation of mass spectrometric data using principal component analysis for determination of the effects of organic lakes on protein binder identification
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26505772
DOI
10.1002/jms.3699
Knihovny.cz E-resources
- Keywords
- artificial ageing, infrared spectroscopy with Fourier transformation, matrix-assisted laser desorption/ionisation - time of flight mass spectrometry, organic lakes, principal component analysis,
- MeSH
- Principal Component Analysis * MeSH
- Time Factors MeSH
- Insecta chemistry MeSH
- Paintings * MeSH
- Copper chemistry MeSH
- Paint MeSH
- Signal Processing, Computer-Assisted * MeSH
- Proteins analysis MeSH
- Software MeSH
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization methods MeSH
- Spectroscopy, Fourier Transform Infrared MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Copper MeSH
- Proteins MeSH
Matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) mass spectrometry is commonly used for the identification of proteinaceous binders and their mixtures in artworks. The determination of protein binders is based on a comparison between the m/z values of tryptic peptides in the unknown sample and a reference one (egg, casein, animal glues etc.), but this method has greater potential to study changes due to ageing and the influence of organic/inorganic components on protein identification. However, it is necessary to then carry out statistical evaluation on the obtained data. Before now, it has been complicated to routinely convert the mass spectrometric data into a statistical programme, to extract and match the appropriate peaks. Only several 'homemade' computer programmes without user-friendly interfaces are available for these purposes. In this paper, we would like to present our completely new, publically available, non-commercial software, ms-alone and multiMS-toolbox, for principal component analyses of MALDI-TOF MS data for R software, and their application to the study of the influence of heterogeneous matrices (organic lakes) for protein identification. Using this new software, we determined the main factors that influence the protein analyses of artificially aged model mixtures of organic lakes and fish glue, prepared according to historical recipes that were used for book illumination, using MALDI-TOF peptide mass mapping.
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