Import of Fluorescent RNA into Mitochondria of Living Cells
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- 5S-rRNA, Fluorescent in vivo hybridization of mtDNA, Mitochondrial DNA, Mitochondrial nucleoids, Nucleic acid import into mitochondria,
- MeSH
- Hep G2 Cells MeSH
- Fluorescent Dyes MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Humans MeSH
- DNA, Mitochondrial genetics MeSH
- Mitochondria genetics MeSH
- Cell Line, Tumor MeSH
- RNA, Mitochondrial MeSH
- RNA, Ribosomal, 5S genetics MeSH
- RNA genetics MeSH
- Transfection methods MeSH
- RNA Transport genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- DNA, Mitochondrial MeSH
- RNA, Mitochondrial MeSH
- RNA, Ribosomal, 5S MeSH
- RNA MeSH
Methods of in vivo visualization and manipulation of mitochondrial genetic machinery are limited due to the need to surpass not only the cytoplasmic membrane but also two mitochondrial membranes. Here, we employ the matrix-addressing sequence of mitochondrial ribosomal 5S-rRNA (termed MAM), which is naturally imported into mammalian mitochondria, to construct an import system for in vivo targeting of mitochondrial (mt) DNA or mtRNA, in order to provide fluorescence hybridization of the desired sequences.
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