Metabolomic profiling of urinary changes in mice with monosodium glutamate-induced obesity
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26577083
DOI
10.1007/s00216-015-9133-0
PII: 10.1007/s00216-015-9133-0
Knihovny.cz E-resources
- Keywords
- Diabetes, Metabolomics, Monosodium glutamate (MSG) induced obesity, Mouse model, NMR, Urine,
- MeSH
- Sodium Glutamate adverse effects MeSH
- Insulin metabolism MeSH
- Liver metabolism MeSH
- Blood Glucose metabolism MeSH
- Humans MeSH
- Lipid Metabolism MeSH
- Metabolomics * MeSH
- Urine chemistry MeSH
- Mice MeSH
- Obesity etiology genetics metabolism urine MeSH
- Peroxisome Proliferator-Activated Receptors genetics metabolism MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Sodium Glutamate MeSH
- Insulin MeSH
- Blood Glucose MeSH
- Peroxisome Proliferator-Activated Receptors MeSH
Obesity with related complications represents a widespread health problem. The etiopathogenesis of obesity is often studied using numerous rodent models. The mouse model of monosodium glutamate (MSG)-induced obesity was exploited as a model of obesity combined with insulin resistance. The aim of this work was to characterize the metabolic status of MSG mice by NMR-based metabolomics in combination with relevant biochemical and hormonal parameters. NMR analysis of urine at 2, 6, and 9 months revealed altered metabolism of nicotinamide and polyamines, attenuated excretion of major urinary proteins, increased levels of phenylacetylglycine and allantoin, and decreased concentrations of methylamine in urine of MSG-treated mice. Altered levels of creatine, citrate, succinate, and acetate were observed at 2 months of age and approached the values of control mice with aging. The development of obesity and insulin resistance in 6-month-old MSG mice was also accompanied by decreased mRNA expressions of adiponectin, lipogenetic and lipolytic enzymes and peroxisome proliferator-activated receptor-gamma in fat while mRNA expressions of lipogenetic enzymes in the liver were enhanced. At the age of 9 months, biochemical parameters of MSG mice were normalized to the values of the controls. This fact pointed to a limited predictive value of biochemical data up to age of 6 months as NMR metabolomics confirmed altered urine metabolic composition even at 9 months.
References provided by Crossref.org
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