Phylogeny and Morphological Variability of Trypanosomes from African Pelomedusid Turtles with Redescription of Trypanosoma mocambicum Pienaar, 1962
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
26599723
DOI
10.1016/j.protis.2015.10.002
PII: S1434-4610(15)00065-6
Knihovny.cz E-resources
- Keywords
- Pelusios, SSU rRNA gene., Trypanosoma, phylogeny, polymorphism, turtle,
- MeSH
- Phylogeny * MeSH
- Molecular Sequence Data MeSH
- RNA, Protozoan genetics MeSH
- Sequence Analysis, DNA veterinary MeSH
- Trypanosoma classification cytology genetics physiology MeSH
- Turtles parasitology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Africa MeSH
- Names of Substances
- RNA, Protozoan MeSH
Little is known about host specificity, genetic diversity and phylogenetic relationships of African turtle trypanosomes. Using PCR targeting the SSU rRNA gene, we detected trypanosomes in 24 of 134 (17.9%) wild caught African pelomedusid turtles: Pelusios upembae (n=14), P. bechuanicus (n=1), P. rhodesianus (n=3) and P. subniger (n=6). Mixed infection of Trypanosoma species was confirmed by PCR in three specimens of P. upembae, and in one specimen each of P. bechuanicus, P. rhodesianus, and P. subniger. Microscopic examination of stained blood smears revealed two distinct forms (broad and slender) of trypomastigotes. The broad form coincided in morphology with T. mocambicumPienaar, 1962. Accordingly, we have designated this form as the neotype of T. mocambicum. In phylogenetic analysis of the SSU rRNA gene, all the new turtle trypanosome sequences grouped in a single clade within the strongly supported "aquatic" clade of Trypanosoma species. The turtle trypanosome clade was further subdivided into two subclades, which did not correlate with host turtle species or trypanosome morphology. This study provides the first sequence data of Trypanosoma species isolated from freshwater turtles from tropical Africa and extends knowledge on diversity of trypanosomes in the Afrotropical zoogeographical realm.
References provided by Crossref.org
Euglenozoa: taxonomy, diversity and ecology, symbioses and viruses
Diversity and evolution of anuran trypanosomes: insights from the study of European species
GENBANK
KP888898, KP888899, KP888900, KP888901, KP888902, KP888903, KP888904, KP888905, KP888906, KP888907, KP888908, KP888909, KP888910, KP888911, KP888912, KP888913, KP888914, KP888915, KP888916, KP888917, KP888918, KP888919, KP888920, KP888921, KP888922
