Prevalence of pathogenic bacteria in Ixodes ricinus ticks in Central Bohemia
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26612395
DOI
10.1007/s10493-015-9988-y
PII: 10.1007/s10493-015-9988-y
Knihovny.cz E-zdroje
- Klíčová slova
- Bacteria, Central Bohemia, Ixodes ricinus, Pathogens, Tick,
- MeSH
- Bacteria klasifikace genetika MeSH
- DNA bakterií genetika MeSH
- fylogeneze MeSH
- klíště růst a vývoj mikrobiologie MeSH
- kočky mikrobiologie parazitologie MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- nymfa mikrobiologie MeSH
- polymerázová řetězová reakce MeSH
- psi mikrobiologie parazitologie MeSH
- RNA ribozomální 16S genetika MeSH
- Spiroplasma klasifikace genetika MeSH
- velkoměsta MeSH
- zvířata MeSH
- Check Tag
- kočky mikrobiologie parazitologie MeSH
- lidé MeSH
- mužské pohlaví MeSH
- psi mikrobiologie parazitologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- velkoměsta MeSH
- Názvy látek
- DNA bakterií MeSH
- RNA ribozomální 16S MeSH
Bacteria associated with the tick Ixodes ricinus were assessed in specimens unattached or attached to the skin of cats, dogs and humans, collected in the Czech Republic. The bacteria were detected by PCR in 97 of 142 pooled samples including 204 ticks, i.e. 1-7 ticks per sample, collected at the same time from one host. A fragment of the bacterial 16S rRNA gene was amplified, cloned and sequenced from 32 randomly selected samples. The most frequent sequences were those related to Candidatus Midichloria midichlori (71% of cloned sequences), followed by Diplorickettsia (13%), Spiroplasma (3%), Rickettsia (3%), Pasteurella (3%), Morganella (3%), Pseudomonas (2%), Bacillus (1%), Methylobacterium (1%) and Phyllobacterium (1%). The phylogenetic analysis of Spiroplasma 16S rRNA gene sequences showed two groups related to Spiroplasma eriocheiris and Spiroplasma melliferum, respectively. Using group-specific primers, the following potentially pathogenic bacteria were detected: Borellia (in 20% of the 142 samples), Rickettsia (12%), Spiroplasma (5%), Diplorickettsia (5%) and Anaplasma (2%). In total, 68% of I. ricinus samples (97/142) contained detectable bacteria and 13% contained two or more putative pathogenic groups. The prevalence of tick-borne bacteria was similar to the observations in other European countries.
Coventry University Coventry CV1 5FB UK
Crop Research Institute Drnovska 507 73 Ruzyne 16106 Prague 6 Czech Republic
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