The Sustainable Release of Vancomycin and Its Degradation Products From Nanostructured Collagen/Hydroxyapatite Composite Layers
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26886321
DOI
10.1016/s0022-3549(15)00175-6
PII: S0022-3549(15)00175-6
Knihovny.cz E-zdroje
- Klíčová slova
- HPLC, anti-infectives, coating, controlled release, degradation products, drug delivery systems, nanoparticles, pharmacokinetics, polymeric drug delivery systems,
- MeSH
- hydroxyapatit MeSH
- karbodiimidy chemie MeSH
- kolagen chemie MeSH
- léky s prodlouženým účinkem chemie MeSH
- methylaminy chemie MeSH
- nanočástice chemie MeSH
- nanostruktury chemie MeSH
- nosiče léků chemie MeSH
- systémy cílené aplikace léků metody MeSH
- vankomycin chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1-(3-dimethylaminopropyl)-dimethylaminopropyl-ethylcarbodiimide hydrochloride MeSH Prohlížeč
- hydroxyapatit MeSH
- karbodiimidy MeSH
- kolagen MeSH
- léky s prodlouženým účinkem MeSH
- methylaminy MeSH
- nosiče léků MeSH
- vankomycin MeSH
Infections of the musculoskeletal system present a serious problem with regard to the field of orthopedic and trauma medicine. The aim of the experiment described in this study was to develop a resorbable nanostructured composite layer with the controlled elution of antibiotics. The layer is composed of collagen, hydroxyapatite nanoparticles, and vancomycin hydrochloride (10 wt%). The stability of the collagen was enhanced by means of cross-linking. Four cross-linking agents were studied, namely an ethanol solution, a phosphate buffer solution of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide, genipin, and nordihydroguaiaretic acid. High performance liquid chromatography was used so as to characterize the in vitro release rates of the vancomycin and its crystalline degradation antibiotically inactive products over a 21-day period. The maximum concentration of the released active form of vancomycin (approximately 265 mg/L) exceeded the minimum inhibitory concentration up to an order of 17 times without triggering the burst releasing effect. At the end of the experiment, the minimum inhibitory concentration was exceeded by up to 6 times (approximately 100 mg/L). It was determined that the modification of collagen with hydroxyapatite nanoparticles does not negatively influence the sustainable release of vancomycin. The balance of vancomycin and its degradation products was observed after 14 days of incubation.
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