Effects of Icodextrin and Glucose Bicarbonate/Lactate-Buffered Peritoneal Dialysis Fluids on Effluent Cell Population and Biocompatibility Markers IL-6 and CA125 in Incident Peritoneal Dialysis Patients
Jazyk angličtina Země Austrálie Médium print-electronic
Typ dokumentu časopisecké články
PubMed
26929256
DOI
10.1111/1744-9987.12391
Knihovny.cz E-zdroje
- Klíčová slova
- Biocompatibility, Cancer antigen 125, Icodextrin interleukin-6, Peritoneal dialysis,
- MeSH
- antigen CA-125 metabolismus MeSH
- dialyzační roztoky chemie MeSH
- dospělí MeSH
- glukany chemie MeSH
- glukosa chemie MeSH
- hydrogenuhličitany chemie MeSH
- icodextrin MeSH
- interleukin-6 metabolismus MeSH
- laktáty chemie MeSH
- lidé středního věku MeSH
- lidé MeSH
- peritoneální dialýza metody MeSH
- počet leukocytů MeSH
- prospektivní studie MeSH
- průtoková cytometrie MeSH
- senioři MeSH
- zánět MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antigen CA-125 MeSH
- dialyzační roztoky MeSH
- glukany MeSH
- glukosa MeSH
- hydrogenuhličitany MeSH
- icodextrin MeSH
- interleukin-6 MeSH
- laktáty MeSH
Icodextrin peritoneal dialysis (PD) solution has been shown to increase interleukin-6 (IL-6) levels in PD effluent as well as leukocyte and mesothelial cell count. Mesothelial cells release cancer antigen 125 (CA125), which is used as a marker of mesothelial cell mass. This 1-year prospective study was designed to compare peritoneal effluent cell population, its inflammatory phenotype and biocompatibility biomarkers IL-6 and CA125 between icodextrin (E) and glucose bicarbonate/lactate (P) based PD solutions. Using baseline peritoneal ultrafiltration capacity, 19 stable incident PD patients were allocated either to P only (N = 8) or to P plus E for the overnight dwell (N = 11). Flow cytometry was used to measure white blood cell count and differential and the expression of inflammatory molecules on peritoneal cells isolated from timed overnight peritoneal effluents. Compared to P, E effluent showed higher leukocyte (10.9 vs. 7.9), macrophages (6.1 vs. 2.5) and mesothelial cells (0.3 vs. 0.1)×10(6) /L count, as well as expression of HLA DR on mesothelial cells and IL-6 (320.5 vs. 141.2 pg/min) on mesothelial cells and CA125 appearance rate (159.6 vs. 84.3 IU/min), all P < 0.05. In the E group, correlation between IL-6 and CA125 effluent levels (r = 0.503, P < 0.05) as well as appearance rates (r = 0.774, P < 0.001) was demonstrated. No effect on systemic inflammatory markers or peritoneal permeability was found. Icodextrin PD solution activates local inflammation without systemic consequences so the clinical relevance of this observation remains obscure. Correlation between effluent IL-6 and CA125 suggests that CA125 might be upregulated due to inflammation and thus is not a reliable marker of mesothelial cell mass and/or biocompatibility.
Biochemistry Charles University Medical School and Teaching Hospital Plzen Plzen Czech Republic
Biomedical Centre Faculty of Medicine in Plzen Charles University Prague Plzen Czech Republic
Hematooncology Charles University Medical School and Teaching Hospital Plzen Plzen Czech Republic
Nuclear Medicine Charles University Medical School and Teaching Hospital Plzen Plzen Czech Republic
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