Silver nanoparticles strongly enhance and restore bactericidal activity of inactive antibiotics against multiresistant Enterobacteriaceae
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
26970828
DOI
10.1016/j.colsurfb.2016.03.007
PII: S0927-7765(16)30151-5
Knihovny.cz E-zdroje
- Klíčová slova
- Antibiotic, Cytotoxicity, Resistant bacteria, Silver nanoparticle, Synergism,
- MeSH
- antibakteriální látky farmakologie MeSH
- beta-laktamasy genetika metabolismus MeSH
- cefotaxim farmakologie MeSH
- ceftazidim farmakologie MeSH
- ciprofloxacin farmakologie MeSH
- Escherichia coli účinky léků enzymologie genetika růst a vývoj MeSH
- exprese genu MeSH
- gentamiciny farmakologie MeSH
- Klebsiella pneumoniae účinky léků enzymologie genetika růst a vývoj MeSH
- kovové nanočástice chemie toxicita MeSH
- meropenem MeSH
- mikrobiální testy citlivosti MeSH
- mnohočetná bakteriální léková rezistence účinky léků genetika MeSH
- stříbro farmakologie MeSH
- synergismus léků MeSH
- thienamyciny farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibakteriální látky MeSH
- beta-laktamasy MeSH
- cefotaxim MeSH
- ceftazidim MeSH
- ciprofloxacin MeSH
- gentamiciny MeSH
- meropenem MeSH
- stříbro MeSH
- thienamyciny MeSH
Bacterial resistance to conventional antibiotics is currently one of the most important healthcare issues, and has serious negative impacts on medical practice. This study presents a potential solution to this problem, using the strong synergistic effects of antibiotics combined with silver nanoparticles (NPs). Silver NPs inhibit bacterial growth via a multilevel mode of antibacterial action at concentrations ranging from a few ppm to tens of ppm. Silver NPs strongly enhanced antibacterial activity against multiresistant, β-lactamase and carbapenemase-producing Enterobacteriaceae when combined with the following antibiotics: cefotaxime, ceftazidime, meropenem, ciprofloxacin and gentamicin. All the antibiotics, when combined with silver NPs, showed enhanced antibacterial activity at concentrations far below the minimum inhibitory concentrations (tenths to hundredths of one ppm) of individual antibiotics and silver NPs. The enhanced activity of antibiotics combined with silver NPs, especially meropenem, was weaker against non-resistant bacteria than against resistant bacteria. The double disk synergy test showed that bacteria produced no β-lactamase when treated with antibiotics combined with silver NPs. Low silver concentrations were required for effective enhancement of antibacterial activity against multiresistant bacteria. These low silver concentrations showed no cytotoxic effect towards mammalian cells, an important feature for potential medical applications.
Citace poskytuje Crossref.org
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